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Am J Physiol Renal Physiol (October 22, 2002). doi:10.1152/ajprenal.00235.2002
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Articles in PresS, published online ahead of print October 22, 2002
Am J Physiol Renal Physiol, 10.1152/ajprenal.00235.2002
Submitted on June 24, 2002
Accepted on September 30, 2002

Differential, inducible gene targeting in renal epithelia, vascular endothelium, and visceral organs of Mx1Cre mice

Andre Schneider1, Yahua Zhang2, YouFei Guan2, Linda S. Davis2, and Matthew D. Breyer3*

1 Department of Medicine, Universitatsklinikum Eppendorf, Hamburg, Germany
2 Department of Medicine, Vanderbilt University, Nashville, TN, USA
3 Department of Medicine, Vanderbilt University, Nashville, TN, USA; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA

* To whom correspondence should be addressed. E-mail: matthew.breyer{at}vanderbilt.edu.

The Cre/loxP transgenic system may be used to achieve temporally and/or spatially regulated gene deletion. The Mx1 Cre mouse expresses Cre recombinase under control of the interferon inducible Mx1 promoter. Mx1 Cre mice were crossed with a reporter strain (ROSA26tm1Sor) in which ß-galactosidase activity is expressed only after Cre mediated recombination, to determine the cellular pattern of Cre mediated genetic recombination in the kidney and other tissues. Widespread recombination was observed in vascular endothelium as well as in the liver and spleen. Recombination was restricted to subsets of stromal cells in uterus, duodenum, colon, aorta and kidney. In the cortex, X-gal activity was detected in a subset of tubules and all glomerular cells, including endothelium, mesangium, and podocytes. No X-gal activity was detected in proximal tubules. Co-staining of kidneys with segment specific markers demonstrated induction of X-gal activity in collecting duct, with sporadic labeling of the thick ascending limb but no significant labeling of distal convoluted tubules. We conclude that Mx1-driven gene recombination is spatially as well as temporally restricted. The Mx1Cre transgene should prove a useful reagent to achieve temporally regulated recombination in endothelial, glomerular and distal renal epithelia in mice.




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