| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print April 30, 2002
Am J Physiol Renal Physiol, 10.1152/ajprenal.00236.2001
Submitted on July 30, 2001
Accepted on April 4, 2002
1 Department of Cell Biology, Vanderbilt Universtiy, Nashville, TN, USA
2 Department of Medicine, Vanderbilt University, Nashville, TN, USA
* To whom correspondence should be addressed. E-mail: jim.mckanna{at}mcmail.vanderbilt.edu.
The renal inner medulla and its distal one-third, the papilla, are major sites of prostanoids synthesis involved in water and electrolyte homeostasis. These sites contain variable levels of cyclooxygenase-2 (COX-2), a key prostaglandin synthase enzyme that is sensitive to adrenal steroids. Immunoreactive renal medullary COX-2, restricted to interstitial cells in control adult rats, shows a gradient of intense staining at the tip of the papilla that gradually diminishes to undetectable levels in the proximal inner medulla. We used adrenalectomy (ADX) and steroid replacement to investigate the effects of steroids on papillary COX-2. Immunoblots demonstrate that papillary COX-2 was reduced by half after 2wk ADX; glucocorticoid replacement ameliorated the decline but not to control levels. Mineralocorticoid (DOCA) replacement stimulated papillary COX-2 >5-fold over control; both the intensity of immunostaining and the numbers of COX-2-positive cells in the inner medulla increased. Similar stimulation of papillary COX-2 resulted from DOCA treatment of normal control rats, but the response was blunted in rats fed a low-salt diet and absent in Brattleboro rats. DOCA treatment of mouse renal medullary interstitial cells in culture had no effect; but increased tonicity of the culture medium with NaCl caused strong upregulation of COX-2. Urea, a permeant molecule, had no effect. Together, these results suggest that mineralocorticoids lead to upregulation of COX-2 in rat renal medulla by indirect pathways, probably involving induced electrolyte hypertonicity in the interstitial fluid.
This article has been cited by other articles:
|
|
 |
|
  B. Yao, R. C. Harris, and M.-Z. Zhang Intrarenal Dopamine Attenuates Deoxycorticosterone Acetate/High Salt-Induced Blood Pressure Elevation in Part Through Activation of a Medullary Cyclooxygenase 2 Pathway Hypertension, November 1, 2009; 54(5): 1077 - 1083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Brennan, S. Kaufman, S. W. Reynolds, B. T. McCook, G. Kan, I. Christiaens, M. E. Symonds, and D. M. Olson Differential effects of maternal nutrient restriction through pregnancy on kidney development and later blood pressure control in the resulting offspring Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2008; 295(1): R197 - R205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yao, J. Xu, R. C. Harris, and M.-Z. Zhang Renal localization and regulation of 15-hydroxyprostaglandin dehydrogenase Am J Physiol Renal Physiol, February 1, 2008; 294(2): F433 - F439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Yao, R. C. Harris, and M.-Z. Zhang Interactions between 11{beta}-hydroxysteroid dehydrogenase and COX-2 in kidney Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1767 - R1773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chen, M. Zhao, R. Rao, H. Inoue, and C.-M. Hao C/EBP{beta} and Its Binding Element Are Required for NF{kappa}B-induced COX2 Expression Following Hypertonic Stress J. Biol. Chem., April 22, 2005; 280(16): 16354 - 16359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-Z. Zhang, B. Yao, J. A. McKanna, and R. C. Harris Cross talk between the intrarenal dopaminergic and cyclooxygenase-2 systems Am J Physiol Renal Physiol, April 1, 2005; 288(4): F840 - F845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Madsen, J. Stubbe, T. Yang, O. Skott, S. Bachmann, and B. L. Jensen Low endogenous glucocorticoid allows induction of kidney cortical cyclooxygenase-2 during postnatal rat development Am J Physiol Renal Physiol, January 1, 2004; 286(1): F26 - F37. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-Z. Zhang, S.-w. Wang, H. Cheng, Y. Zhang, J. A. McKanna, and R. C. Harris Regulation of renal cortical cyclooxygenase-2 in young rats Am J Physiol Renal Physiol, November 1, 2003; 285(5): F881 - F888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Campean, F. Theilig, A. Paliege, M. Breyer, and S. Bachmann Key enzymes for renal prostaglandin synthesis: site-specific expression in rodent kidney (rat, mouse) Am J Physiol Renal Physiol, July 1, 2003; 285(1): F19 - F32. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
