AJP - Renal Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (December 10, 2002). doi:10.1152/ajprenal.00238.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/4/F812    most recent
00238.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by BELFODIL, R.
Right arrow Articles by POUJEOL, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by BELFODIL, R.
Right arrow Articles by POUJEOL, P.

Articles in PresS, published online ahead of print December 10, 2002
Am J Physiol Renal Physiol, 10.1152/ajprenal.00238.2002
Submitted on June 26, 2002
Accepted on December 3, 2002

CFTR DEPENDENT AND INDEPENDENT SWELLING-ACTIVATED K+ CURRENTS IN PRIMARY CULTURES OF MOUSE NEPHRON

RADIA BELFODIL1, HERVE BARRIERE1, ISABELLE RUBERA1, MICHEL TAUC1, CHANTAL POUJEOL1, MICHEL BIDET1, and PHILIPPE POUJEOL1*

1 UMR 6548, CNRS, Universite de Nice-Sophia Antipolis, NICE CEDEX 2, France

* To whom correspondence should be addressed. E-mail: poujeol{at}unice.fr.

The role of CFTR in the control of K+ currents was studied in mouse kidney. Using whole cell clamp, K+ currents were identified on the basis pharmacological sensitivities in primary cultures of proximal, distal convoluted (DCT) and cortical collecting tubule (CCT) from wild type (WT) and cftr knock-out (KO) mice. In DCT and CCT cells, forskolin activated a 293B-sensitive K+ current in WT but not in KO mice. In these cells, a hypotonic shock induced K+ currents blocked by charybdotoxin in WT but not in KO mice. In PCT cells from WT and KO mice, the hypotonicity-induced K+ currents were insensitive to these toxins and were activated at extracellular pH 8.0 and inhibited at pH 6.0 suggesting that the corresponding channel was TASK2. In conclusion CFTR is implicated in the control of KCNQ1 and Ca2+-sensitive swelling K+ conductances in DCT and CCT but not in PCT cells. In KO mice the impairment of RVD process in DCT and CCT could be due to the loss of an autocrine mechanism implicating ATP and adenosine which controls both swelling Cl- and K+ channels.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
S. L'Hoste, H. Barriere, R. Belfodil, I. Rubera, C. Duranton, M. Tauc, C. Poujeol, J. Barhanin, and P. Poujeol
Extracellular pH alkalinization by Cl-/HCO3- exchanger is crucial for TASK2 activation by hypotonic shock in proximal cell lines from mouse kidney
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F628 - F638.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. L. Pluznick and S. C. Sansom
BK channels in the kidney: role in K+ secretion and localization of molecular components
Am J Physiol Renal Physiol, September 1, 2006; 291(3): F517 - F529.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. L. Pluznick, P. Wei, P. R. Grimm, and S. C. Sansom
BK-{beta}1 subunit: immunolocalization in the mammalian connecting tubule and its role in the kaliuretic response to volume expansion
Am J Physiol Renal Physiol, April 1, 2005; 288(4): F846 - F854.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
H. Barriere, R. Belfodil, I. Rubera, M. Tauc, F. Lesage, C. Poujeol, N. Guy, J. Barhanin, and P. Poujeol
Role of TASK2 Potassium Channels Regarding Volume Regulation in Primary Cultures of Mouse Proximal Tubules
J. Gen. Physiol., July 28, 2003; 122(2): 177 - 190.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.