AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (November 5, 2002). doi:10.1152/ajprenal.00239.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/3/F474    most recent
00239.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, M. K.
Right arrow Articles by Carroll, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, M. K.
Right arrow Articles by Carroll, M. A.

Articles in PresS, published online ahead of print November 5, 2002
Am J Physiol Renal Physiol, 10.1152/ajprenal.00239.2002
Submitted on July 1, 2002
Accepted on October 21, 2002

Renal Arterial 20-Hydroxyeicosatetraenoic Acid Levels: Regulation by Cyclooxygenase

Monica K. Cheng1, John C. McGiff1, and Mairead A. Carroll1*

1 Department of Pharmacology, New York Medical College, Valhalla, NY, USA

* To whom correspondence should be addressed. E-mail: mairead_carroll{at}nymc.edu.

20-hydroxyeicosatetraenoic acid (HETE), a potent vasoconstrictor, is generated by the cytochrome P450 (CYP){omega}-hydroxylases, and is the principal eicosanoid produced by preglomerular microvessels (PGA). It is released from PGA by angiotensin II and is subject to metabolism by cyclooxygenase (COX). As low salt (LS) intake stimulates the renin-angiotensin-system and induces renal cortical COX-2 expression, we examined 20-HETE release from renal arteries (interlobar and arcuate/interlobular arteries) obtained from 6 to 7 week old male Sprague-Dawley rats fed either normal salt (NS; 0.4% NaCl) or LS (0.05% NaCl) diets for 10 days. With NS intake, the levels of 20-HETE recovered was similar in arcuate/interlobular arteries and interlobar arteries: 30.1±8.5 vs. 24.6±5.3 ng/mg protein/30 min, respectively. A LS diet increased 20-HETE levels in the incubate of either arcuate/interlobular or interlobar renal arteries only when COX was inhibited. Addition of indomethacin (10µM) to the incubate of arteries obtained from LS diet fed rats resulted in a two- to three-fold increase in 20-HETE release from arcuate/interlobular arteries, from 39.1±13.2 to 101.8±42.6 ng/mg protein/30 min (p<0.03), and interlobar arteries, from 31.7±15.1 to 61.9±29.4 ng/mg protein/30 min (p<0.05), compared to release of 20-HETE when COX was not inhibited. A LS diet enhanced vascular expression of CYP4A and COX-2 in arcuate/interlobular arteries, COX-1 being unaffected. Metabolism of 20-HETE by COX is proposed to represent an important regulatory mechanism in setting preglomerular microvascular tone.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y.-J. Chen, J. Li, and J. Quilley
Deficient renal 20-HETE release in the diabetic rat is not the result of oxidative stress
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2305 - H2312.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
P. Minuz, H. Jiang, C. Fava, L. Turolo, S. Tacconelli, M. Ricci, P. Patrignani, A. Morganti, A. Lechi, and J. C. McGiff
Altered Release of Cytochrome P450 Metabolites of Arachidonic Acid in Renovascular Disease
Hypertension, May 1, 2008; 51(5): 1379 - 1385.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
X. Fang, F. M. Faraci, T. L. Kaduce, S. Harmon, M. L. Modrick, S. Hu, S. A. Moore, J. R. Falck, N. L. Weintraub, and A. A. Spector
20-Hydroxyeicosatetraenoic acid is a potent dilator of mouse basilar artery: role of cyclooxygenase
Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2301 - H2307.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
E. Joly, R. Seqqat, B. Flamion, N. Caron, A. Michel, J. D. Imig, and R. Kramp
Increased renal vascular reactivity to ANG II after unilateral nephrectomy in the rat involves 20-HETE
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R977 - R986.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. A. Carroll, A. B. Doumad, J. Li, M. K. Cheng, J. R. Falck, and J. C. McGiff
Adenosine2A receptor vasodilation of rat preglomerular microvessels is mediated by EETs that activate the cAMP/PKA pathway
Am J Physiol Renal Physiol, July 1, 2006; 291(1): F155 - F161.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. L. Liclican, J. C. McGiff, P. L. Pedraza, N. R. Ferreri, J. R. Falck, and M. A. Carroll
Exaggerated response to adenosine in kidneys from high salt-fed rats: role of epoxyeicosatrienoic acids
Am J Physiol Renal Physiol, August 1, 2005; 289(2): F386 - F392.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. D. Imig
20-HETE or EETs: which arachidonic acid metabolite regulates proximal tubule transporters and contributes to pressure natriuresis?
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R3 - R5.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.