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1 Department of Cell Biology, Niigata University Graduate School of Medical and Dental Sciences, Institute of Nephrology, Niigata, Niigata, Japan; Department of Pediatrics, The University of Tokyo, Graduate School of Medicine, Bunkyo, Tokyo, Japan
2 Department of Cell Biology, Niigata University Graduate School of Medical and Dental Sciences, Institute of Nephrology, Niigata, Niigata, Japan
3 Department of Pediatrics, The University of Tokyo, Graduate School of Medicine, Bunkyo, Tokyo, Japan
* To whom correspondence should be addressed. E-mail: kawachi{at}med.niigata-u.ac.jp.
Recent investigations have revealed the importance of glomerular podocytes with its diaphragm as the major filtration barrier. Junctional adhesion molecule 4 (JAM4) has been identified as a protein which interacts with MAGI-1, and is reported to be expressed on podocytes. In order to elucidate the role of JAM4 on podocytes, we examined the expression of JAM4 and MAGI-1 in normal and two different proteinuric rat models: puromycin aminonucleoside (PAN) nephropathy and anti-nephrin antibody-induced (ANA) nephropathy, one model with and one without effacement of podocyte foot process. JAM4 was detected by immunoelectron microscopy at the apical membrane of normal podocytes. JAM4 immunostaining was focally increased in the podocytes in PAN nephropathy but not in ANA nephropathy. In proteinuric podocytes, the expression of JAM4 was distinct from that of MAGI-1 or other SD molecules such as nephrin and ZO-1. Close colocalization of JAM4 and ezrin was maintained in PAN nephropathy. By immunoelectron microscopy, the signals for JAM4 were detected at the free apical membrane of the podocytes with effaced foot processes. Studies with selective detergent extract revealed that the subcellular localization of JAM4 was altered in PAN nephropathy. Thus the altered expression of JAM4 appears to be associated with the morphological changes of the podocytes and can be a useful marker of the injured podocytes. JAM4 may have a different role at the apical membrane besides the role as a junctional molecule, and is likely associated with the unique structure of this epithelium.
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