| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, Indiana, USA
2 Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA
* To whom correspondence should be addressed. E-mail: gtanner{at}iupui.edu.
Sulfonefluorescein (SF) is a fluorescent organic anion secreted by kidney proximal tubules. The purposes of this study were 1) to quantify accumulation of SF in normal and cystic rat kidneys in vivo and 2) to test whether SF accumulation could be used as a marker for cysts derived from proximal tubules. Male Munich-Wistar rats, normal Han:SPRD rats, and heterozygous Han:SPRD rats with autosomal-dominant polycystic kidney disease were anesthetized with Inactin and solutions containing SF were administered by constant i.v. infusion. In Munich-Wistar rats, SF fluorescence in the urinary space of Bowman's capsule averaged 0.15 ± 0.04 (n = 17) times that of glomerular capillary plasma, consistent with extensive plasma protein binding of SF. In normal Han:SPRD rats, steady-state cell cytoplasm SF fluorescence in proximal tubule and distal tubule cells averaged, respectively, 2.7 ± 1.4 (n = 99 tubules) and 0.2 ± 0.2 (n = 17) times that of peritubular capillary plasma. No punctate SF fluorescence was seen in proximal tubule cell cytoplasm. Probenecid reduced proximal tubule cell SF fluorescence to 0.64 ± 0.40 (n = 64) times that of plasma. Ureteral obstruction decreased the proximal tubule cell-to-lumen SF fluorescence gradient, suggesting that tubule fluid flow normally sweeps away secreted SF. In cystic kidneys, cysts derived from proximal tubules could be identified by their uptake of SF, but cell uptake was patchy. We conclude that in vivo two-photon microscopy is a powerful tool for quantifying glomerular and tubular handling of SF, and SF can be used to identify proximal tubule-derived cysts.
This article has been cited by other articles:
|
|
 |
|
  W. Yu, R. M. Sandoval, and B. A. Molitoris Rapid determination of renal filtration function using an optical ratiometric imaging approach Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1873 - F1880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Kang, I. Toma, A. Sipos, F. McCulloch, and J. Peti-Peterdi Quantitative imaging of basic functions in renal (patho)physiology Am J Physiol Renal Physiol, August 1, 2006; 291(2): F495 - F502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Yu, R. M. Sandoval, and B. A. Molitoris Quantitative intravital microscopy using a Generalized Polarity concept for kidney studies Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1197 - C1208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Molitoris and R. M. Sandoval Intravital multiphoton microscopy of dynamic renal processes Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1084 - F1089. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
