AJP - Renal Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (April 20, 2004). doi:10.1152/ajprenal.00265.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
287/3/F404    most recent
00265.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lerolle, N.
Right arrow Articles by Houillier, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lerolle, N.
Right arrow Articles by Houillier, P.
Submitted on July 25, 2003
Accepted on April 12, 2004

Angiotensin II inhibits NaCl absorption in the rat medullary thick ascending limb

Nicolas Lerolle1, Soline Bourgeois1, Francoise Leviel1, Gaetan Lebrun1, Michel Paillard1, and Pascal Houillier1*

1 INSERM Unite 356, Institut Federatif de Recherche 58, Paris, France; Department of Physiology, Universite Rene Descartes et Pierre et Marie Curie, Paris, France; Department of Physiology, Hopital Europeen Georges Pompidou, Paris, France

* To whom correspondence should be addressed. E-mail: pascal.houillier{at}egp.ap-hop-paris.fr.

NaCl reabsorption in the medullary thick ascending limb of Henle (MTALH) contributes to NaCl balance and is, also, responsible for the creation of the medullary interstitial hypertonicity. Despite the presence of AT1 receptors in both the luminal and the basolateral plasma membranes of the MTALH cells, no information is available on the effect of angiotensin II on NaCl reabsorption in MTALH and, further, on the angiotensin II-dependent medullary interstitial osmolality. MTALH from male Sprague-Dawley rats were isolated and microperfused in vitro; transepithelial net chloride absorption (JCl) as well as transepithelial voltage (Vte) were measured. Luminal or peritubular 10-11 and 10-10 M angiotensin II had no effect on JCl or Vte. However, 10-8 M luminal or peritubular angiotensin II reversibly decreased both JCl and Vte. The effect of both luminal and peritubular angiotensin II was prevented by the presence of Losartan (10-6 M). By contrast, PD123319, an AT2 receptor antagonist, did not alter the inhibitory effect of 10-8 M angiotensin II. Finally, no additive effect of luminal and peritubular angiotensin II was observed. We conclude that both luminal and peritubular angiotensin II inhibit NaCl absorption in the MTALH via AT1 receptors. Because of intrarenal angiotensin II synthesis, angiotensin II concentration in medullary tubular and interstitial fluids may be similar in vivo to the concentration that displays an inhibitory effect on NaCl reabsorption under the present experimental conditions.




This article has been cited by other articles:


Home page
 HypertensionHome page
G. B. Silva and J. L. Garvin
Angiotensin II-Dependent Hypertension Increases Na Transport-Related Oxygen Consumption by the Thick Ascending Limb
Hypertension, December 1, 2008; 52(6): 1091 - 1098.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S.D. Crowley, S.B. Gurley, M.J. Herrera, P. Ruiz, R. Griffiths, A.P. Kumar, H.-S. Kim, O. Smithies, T.H. Le, T.M. Coffman, et al.
Role of Renal Angiotensin II Type 1 Receptors in the Genesis of Hypertension: Guyton Revisited: Angiotensin II Causes Hypertension and Cardiac Hypertrophy through Its Receptors in the Kidney. Proc Natl Acad Sci U S A 103: 17985-17990, 2006
J. Am. Soc. Nephrol., February 1, 2007; 18(2): 356 - 363.
[Full Text] [PDF]

Home page
 PhysiologyHome page
W. Neuhofer and F.-X. Beck
Survival in Hostile Environments: Strategies of Renal Medullary Cells
Physiology, June 1, 2006; 21(3): 171 - 180.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.