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Am J Physiol Renal Physiol (December 27, 2002). doi:10.1152/ajprenal.00274.2002
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Submitted on July 29, 2002
Accepted on November 29, 2002

Effect of partial outlet obstruction on rabbit urinary bladder smooth muscle function

Xiaoling Su1, Raymund Stein2, Michaela C. Stanton1, Stephen Zderic2, and Robert S. Moreland1*

1 Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, PA, USA
2 Department of Urology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA

* To whom correspondence should be addressed. E-mail: robert.moreland{at}drexel.edu.

Bladder outlet obstruction secondary to benign prostate hyperplasia is associated with many cellular changes. This study was designed to determine if these changes involve the contractile apparatus. Bladder smooth muscle from rabbits subjected to partial outlet obstruction for two weeks were mounted for isometric force, isotonic shortening velocity, and myosin light chain (MLC) phosphorylation levels. Muscle strips from obstructed bladders exhibited spontaneous phasic activity; muscle strips from control bladders did not. Muscle strips from obstructed bladders exhibited increased sensitivity and higher levels of stress in response to the cumulative addition of KCl or carbachol as compared to control. During non-cumulative addition of KCl or carbachol no differences in sensitivity were noted. Muscle strips from obstructed bladders had elevated basal MLC phosphorylation levels and stimulation produced small increases in MLC phosphorylation as compared to control. Vmax during KCl stimulation of muscle strips from obstructed bladders was 10-fold lower than control. Our results suggest that bladder outlet obstruction produces a muscle cell that develops higher levels of force but with greatly reduced crossbridge cycling rates.




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