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Am J Physiol Renal Physiol (March 5, 2002). doi:10.1152/ajprenal.00324.2001
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Articles in PresS, published online ahead of print March 5, 2002
Am J Physiol Renal Physiol, 10.1152/ajprenal.00324.2001
Submitted on October 26, 2001
Accepted on February 27, 2002

Effects of fatty acids on mitochondrial ß-oxidation gene expression in renal cell lines

Fetta Ouali1, Fatima Djouadi1, and Jean Bastin1*

1 Universite Paris 7, INSERM U319, Paris, France

* To whom correspondence should be addressed. E-mail: bastin{at}necker.fr.

Regulatory effects of fatty acids on gene expression of medium-chain-acylCoA dehydrogenase (MCAD), a mitochondrial ß-oxidation enzyme, were investigated in rabbit kidney cell lines derived from proximal tubule (RC.SV1), thick ascending limb of Henle loop (RC.SV2) or from collecting duct (RC.SV3). Exposure to long-chain fatty acids led to significant increases (x2) in MCAD mRNA abundance in RC.SV1 and RC.SV2 cells; kinetics and dose-response studies established that maximal MCAD gene stimulation was reached 4 hours after addition of 50 µM oleate (C18:1) in the culture medium. These effects of fatty acids were totally abolished in the presence of 1µg/ml actinomycin D, a transcription inhibitor. Staining of cellular lipids revealed that fatty acid-induced gene stimulation could occur in the absence of cellular fatty acid storage. Altogether, these data indicate that small changes in cellular fatty acid flux can have direct short-term effects on fatty acid oxidation enzyme gene expression in renal cells, and this might take part in the regulation of cellular fatty acid homeostasis in response to changes in tubular fluid composition.






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