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1 Division of Nephrology, Department of Medicine, Vanderbilt University, Nashville, TN, USA
2 Department of Pathology, Vanderbilt University, Nashville, TN, USA
3 Division of Nephrology, Department of Medicine, Vanderbilt University, Nashville, TN, USA; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA; Department of Medicine, Veterans Administration Medical Center, Nashville, TN, USA
* To whom correspondence should be addressed. E-mail: zhonghua.qi{at}vanderbilt.edu.
Two non-radioactive methods for determining glomerular filtration rate (GFR) in conscious mice using fluorescein isothiocyanate labeled inulin (FITC-inulin) were evaluated. The first method measured GFR using clearance kinetics of plasma FITC-inulin following a single bolus injection. Based on a two-compartment model, estimated GFR was 236.69 ± 16.55 and 140.20 ± 22.27 µl/min in male and female C57BL/6J mice, respectively. Total or 5/6 nephrectomy reduced inulin clearance to zero or 32.80 ± 9.32 µl /min, respectively. Conversely, diabetes mellitus induced by streptozotocin (STZ) was associated with increased GFR. The other approach measured urinary Inulin clearance using intraperitoneal micro osmotic pumps to delivery FITC-inulin and metabolic cages to collect timed urine samples. This approach yielded similar GFR values of 211.11 ± 26.56 and 157.36 ± 20.02µl/min in male and female mice, respectively. These studies demonstrate the feasibility of repeated non-isotopic measurement of inulin clearance in conscious mice.
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