AJP - Renal Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (November 9, 2004). doi:10.1152/ajprenal.00330.2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
288/3/F568    most recent
00330.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Spurgeon, K. S.
Right arrow Articles by Basile, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spurgeon, K. S.
Right arrow Articles by Basile, D. P.
Submitted on September 1, 2004
Accepted on November 2, 2004

Transforming growth factor-{beta} in acute renal failure: receptor expression, effects on proliferation, cellularity and vascularization after recovery from injury

Kimberly S. Spurgeon1, Deborah L. Donohoe1, and David P. Basile1*

1 Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA

* To whom correspondence should be addressed. E-mail: dbasile{at}mcw.edu.

TGF-{beta}1, and a number of TGF-{beta} responsive genes are transiently enhanced following induction of ischemic acute renal failure in rat. The mRNA and protein expression of TGF-{beta} receptors were analyzed in post-ischemic rat kidneys by ribonuclease protection, in situ hybridization and immunohistochemistry. TGF-{beta}RI and RII were enhanced within 3 days of I/R injury and remained elevated for up 7 days post-I/R; TGF-{beta} receptor expression was localized primarily in regenerating tubules within the outer medulla. A neutralizing TGF-{beta} antibody exacerbated cellular proliferation observed on day-3 post-ischemia, but had no effect on day 1 or 2. TGF-{beta} antibody treatment had no measurable effect on loss of renal function or the restoration of renal function during the recovery response for up to 35 days post-surgery. However, ischemic injury resulted in modest renal hypertrophy that is due, in part, to in an increase in the number of interstitial cells in the post-ischemic kidney. Immunohistochemistry showed that several of these cells stained positively for the fibroblast specific marker, S100A4- positive. Anti-TGF-{beta} treatment substantially attenuated the renal hypertrophy, interstitial cellularity, and S100A4 positive cells present at 35 days post-I/R. Finally, TGF-{beta} immunoneutralization attenuated the loss of renal vascular density following recovery from I/R injury. These data suggest that the TGF-{beta}/ T{beta}R system is enhanced in the post-ischemic kidney. However, the current study failed to identify a prominent role for this system in the repair of proximal tubules following ARF. In contrast, the activation of this system may play an important role in the long-term structure of the post-ischemic kidney by influencing microvascular structure and interstitial cellularity.




This article has been cited by other articles:


Home page
 JAMAHome page
R. Wald, R. R. Quinn, J. Luo, P. Li, D. C. Scales, M. M. Mamdani, J. G. Ray, and for the University of Toronto Acute Kidney Injury
Chronic Dialysis and Death Among Survivors of Acute Kidney Injury Requiring Dialysis
JAMA, September 16, 2009; 302(11): 1179 - 1185.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. A. Zager and A. C. M. Johnson
Renal ischemia-reperfusion injury upregulates histone-modifying enzyme systems and alters histone expression at proinflammatory/profibrotic genes
Am J Physiol Renal Physiol, May 1, 2009; 296(5): F1032 - F1041.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. Geng, R. Lan, G. Wang, A. R. Siddiqi, M. C. Naski, A. I. Brooks, J. L. Barnes, P. Saikumar, J. M. Weinberg, and M. A. Venkatachalam
Inhibition of Autoregulated TGF{beta} Signaling Simultaneously Enhances Proliferation and Differentiation of Kidney Epithelium and Promotes Repair Following Renal Ischemia
Am. J. Pathol., April 1, 2009; 174(4): 1291 - 1308.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
E. C. Leonard, J. L. Friedrich, and D. P. Basile
VEGF-121 preserves renal microvessel structure and ameliorates secondary renal disease following acute kidney injury
Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1648 - F1657.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Schmitt and L. G. Cantley
The impact of aging on kidney repair
Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1265 - F1272.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. P. Basile, K. Fredrich, B. Chelladurai, E. C. Leonard, and A. R. Parrish
Renal ischemia reperfusion inhibits VEGF expression and induces ADAMTS-1, a novel VEGF inhibitor
Am J Physiol Renal Physiol, April 1, 2008; 294(4): F928 - F936.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. R. Spurgeon-Pechman, D. L. Donohoe, D. L. Mattson, H. Lund, L. James, and D. P. Basile
Recovery from acute renal failure predisposes hypertension and secondary renal disease in response to elevated sodium
Am J Physiol Renal Physiol, July 1, 2007; 293(1): F269 - F278.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
Y. Ishii, S. Fujimoto, and T. Fukuda
Gefitinib Prevents Bleomycin-induced Lung Fibrosis in Mice
Am. J. Respir. Crit. Care Med., September 1, 2006; 174(5): 550 - 556.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. P. Basile, D. L. Donohoe, S. A. Phillips, and J. C. Frisbee
Enhanced skeletal muscle arteriolar reactivity to ANG II after recovery from ischemic acute renal failure
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1770 - R1776.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. P. Basile, K. Fredrich, M. Alausa, C. P. Vio, M. Liang, M. R. Rieder, A. S. Greene, and A. W. Cowley Jr.
Identification of persistently altered gene expression in the kidney after functional recovery from ischemic acute renal failure
Am J Physiol Renal Physiol, May 1, 2005; 288(5): F953 - F963.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.