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Am J Physiol Renal Physiol (November 13, 2001). doi:10.1152/ajprenal.00340.2000
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Articles in PresS, published online ahead of print November 13, 2001
Am J Physiol Renal Physiol, 10.1152/ajprenal.00340.2000
Submitted on December 14, 2000
Accepted on October 30, 2001

Immunomorphometric Study of Rat Renal Inner Medulla

Raymond Mejia1* and James B Wade2

1 Mathematical Research Branch, National Institutes of Health, Bethesda, MD, USA
2 Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, USA

* To whom correspondence should be addressed. E-mail: ray{at}helix.nih.gov.

We utilize triple immunofiuorescence immunolabeling of renal tissue sections to identify and count tubules at specified depths of the rat renal inner medulla. We use primary antibodies to aquaporin-1 (AQP1, labeling thin descending limbs, DTL), aquaporin-2 (AQP2, labeling inner medullary collecting ducts, JMCD), chloride channel ClC (labeling thin ascending limbs, ATL), and von Willebrand Factor (labeling descending vasa recta, DVR). Secondary antibodies conjugated to different fiuorophores are used giving a three color display. Labeled structures are then identified and counted. We conclude that at distinct axial distances within the inner medulla a fraction of DTL express AQP1 while others express ClC. Mathematical models can use this data to explore implications for the urine concentrating mechanism.




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