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1 Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, USA
2 Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, USA
3 Department of Veteran Affairs Medical Center, Gainesville, FL, USA
* To whom correspondence should be addressed. E-mail: bcain{at}biochem.med.ufl.edu.
The mineralocorticoid aldosterone is a major regulator of Na+ and acid-base balance and control of blood pressure. Although the long-term effects of aldosterone have been extensively studied, the early aldosterone-responsive genes remain largely unknown. Using DNA array technology, we have characterized changes in gene expression following 1 h of exposure to aldosterone in a mouse inner medullary collecting duct cell line, mIMCD-3. Results from three independent microarray experiments revealed that the expression of many transcripts was affected by aldosterone treatment. Northern blot analysis confirmed the up-regulation of four distinct transcripts identified by the microarray analysis, namely, sgk, CTGF, period homolog, and preproendothelin. Immunoblot analysis for preproendothelin demonstrated increased protein expression. Following the levels of the four transcripts over time showed that each had a unique pattern of expression, suggesting that the cellular response to aldosterone is complex. The results presented here represent a novel list of early aldosterone-responsive transcripts and provide new avenues for elucidating the mechanism of acute aldosterone action in the kidney.
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