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Articles in PresS, published online ahead of print June 11, 2002
Am J Physiol Renal Physiol, 10.1152/ajprenal.00359.2001
Submitted on December 7, 2001
Accepted on June 5, 2002
1 Institut National de la Transfusion Sanguine, INSERM U76, Paris, France; INTS, Paris, France
2 UMR 7519, CNRS, Strasbourg, France
3 Institut National de la Transfusion Sanguine, INSERM U76, Paris, France; U76, INSERM, Paris, France
* To whom correspondence should be addressed. E-mail: trinh{at}idf.inserm.fr.
UT-B1 is the facilitated urea transporter of red blood cells (RBCs) and endothelial cells of descending vasa recta in the kidney. Immunoblotting with a polyclonal antibody against the C-ter sequence of rat UT-B1 revealed UT-B1 as both non-glycosylated (29 kDa) and N-glycosylated (47.5 and 33 kDa) proteins in RBC membranes, kidney medulla, brain, and bladder in rat. In testis, UT-B1 was expressed only as a non-glycosylated protein of 47.5 kDa. Immunocytochemistry confirmed that UT-B1 location is restricted to descending vasa recta. In brain, UT-B1 protein was found in astrocytes and ependymal cells. Cell bodies and perivascular endfeet of astrocytes were labeled in brain cortex whereas astrocyte cell processes were labeled in corpus callosum. Flow cytometry analysis of RBCs revealed a good cross-reactivity of the antibody with mouse and human UT-B1. UT-B1 protein expression in rat kidney medulla was downregulated, greatly by long-term dDAVP infusion, and moderately by furosemide treatment. This study discloses an uneven distribution of UT-B1 protein within astrocytes, and the regulation of renal UT-B1 protein by antidiuretic hormone.
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