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1 Nutritional Science, Department of Nutrition, School of Medicine, Tokushima University, Tokushima, Japan
2 Institute of Molecular and Cellular Biosciences, Tokyo University, Tokyo, Japan
* To whom correspondence should be addressed. E-mail: miyamoto{at}nutr.med.tokushima-u.ac.jp.
Recent studies suggest that vitamin D may play a role in intestinal Na+-dependent phosphate transport adaptation to variable levels of dietary inorganic phosphate (Pi). Therefore, the goal of current study was to assess Na+-dependent Pi cotransport activity in transgenic mice to determine whether vitamin D is an essential mediator of this process. Intestinal brush-border membrane (BBM) Na+-dependent Pi cotransport activity was significantly decreased in VDR null (VDR (-/-)) mice when compared with wild-type (VDR+/+) mice. While intestinal Na/Pi cotransporter (type IIb) mRNA levels were similar in VDR (-/-) and VDR (+/+) mice, type IIb Na/Pi transporter protein expression was markedly suppressed in VDR (-/-) mice when compared with VDR (+/+) mice. Further, Na/Pi cotransport activity in renal BBM was similar in VDR (-/-) mice and VDR(+/+) mice, but type IIa Na/Pi cotransporter protein expression was decreased in VDR (-/-) mice. Following administration of a low Pi diet, type IIb protein expression was significantly increased in VDR (+/+) and VDR (-/-) mice, and type IIb protein expression was present in the intestinal BBM of the VDR (-/-) mice. These data demonstrate that intestinal Na/Pi cotransport adaptation to a low Pi diet occurs independent of vitamin D.
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