AJP - Renal Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (March 18, 2003). doi:10.1152/ajprenal.00396.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/1/F95    most recent
00396.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gui, Y.
Right arrow Articles by Hollenberg, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gui, Y.
Right arrow Articles by Hollenberg, M. D.
Submitted on November 6, 2002
Accepted on March 11, 2003

Bi-directional Regulation of Renal Hemodynamics by Activation of Proteinase-Activated Receptors(PAR) 1 and 2 in the Isolated Perfused Rat Kidney

Yu Gui1, Rodger Loutzenhiser1, and Morley D. Hollenberg2*

1 Department of Pharmacology and Therapeutics, University of Calgary, Calgary, Alberta, Canada
2 Department of Pharmacology and Therapeutics, University of Calgary, Calgary, Alberta, Canada; Department of Medicine, University of Calgary, Calgary, Alberta, Canada

* To whom correspondence should be addressed. E-mail: mhollenb{at}ucalgary.ca.

Proteinase-activated receptors (PARs) are activated by either serine proteinases or synthetic peptides corresponding to the N-terminal tethered ligand sequences that are unmasked by proteolytic cleavage. Although PARs are highly expressed in the kidney, their roles in regulating renal function are not known. In the present study, we evaluated the impact of PAR activation on renal hemodynamics using PAR1- and PAR2-activating peptides (TFLLR-NH2 and SLIGRL-NH2)and proteinases (thrombin and trypsin) as PAR agonists in the isolated perfused rat kidney preparation. PAR1 activation resulted in renal vasoconstriction and a marked reduction in the glomerular filtration rate (GFR). In contrast, PAR2 activation caused vasodilation, partially reversing the vasoconstriction induced by TFLLR-NH2 and angiotensin II (Ang II), and increasing GFR that had been pre-reduced by Ang II. The vasoconstrictor actions of PAR1 activation were abolished by protein kinase C inhibition. The PAR2-induced vasodilation was only partially blocked by L-NAME, suggesting both NO-dependent and -independent mechanisms. Although PAR4 mRNA was detected in renal parenchyma, the PAR4-activating peptide, AYPGKF-NH2, had no effect on RPF. We conclude that PAR2 and PAR2 play bi-directional roles in the regulation of renal hemodynamics.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Vesey, W. A. Kruger, P. Poronnik, G. C. Gobe, and D. W. Johnson
Proinflammatory and proliferative responses of human proximal tubule cells to PAR-2 activation
Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1441 - F1449.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
N. Nitescu, E. Grimberg, S.-E. Ricksten, N. Marcussen, H. Nordlinder, and G. Guron
Effects of thrombin inhibition with melagatran on renal hemodynamics and function and liver integrity during early endotoxemia
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1117 - R1124.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Wang, J. Breaks, K. Loutzenhiser, and R. Loutzenhiser
Effects of inhibition of the Na+/K+/2Cl- cotransporter on myogenic and angiotensin II responses of the rat afferent arteriole
Am J Physiol Renal Physiol, March 1, 2007; 292(3): F999 - F1006.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Sevastos, S. E. Kennedy, D. R. Davis, M. Sam, P. W. Peake, J. A. Charlesworth, N. Mackman, and J. H. Erlich
Tissue factor deficiency and PAR-1 deficiency are protective against renal ischemia reperfusion injury
Blood, January 15, 2007; 109(2): 577 - 583.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Ayala, D. J. Warejcka, M. Olague-Marchan, and S. S. Twining
Corneal Activation of Prothrombin to Form Thrombin, Independent of Vascular Injury
Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 134 - 143.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Wang, M. D. Hollenberg, and R. Loutzenhiser
Redundant signaling mechanisms contribute to the vasodilatory response of the afferent arteriole to proteinase-activated receptor-2
Am J Physiol Renal Physiol, January 1, 2005; 288(1): F65 - F75.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
A. Kawabata, S. Kubo, Y. Nakaya, T. Ishiki, R. Kuroda, F. Sekiguchi, N. Kawao, and H. Nishikawa
Distinct roles for protease-activated receptors 1 and 2 in vasomotor modulation in rat superior mesenteric artery
Cardiovasc Res, March 1, 2004; 61(4): 683 - 692.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Wang, G. Trottier, and R. Loutzenhiser
Determinants of renal afferent arteriolar actions of bradykinin: evidence that multiple pathways mediate responses attributed to EDHF
Am J Physiol Renal Physiol, September 1, 2003; 285(3): F540 - F549.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.