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1 Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA
2 Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA; Department of Cell Biology & Physiology, University of Pittsburgh, Pittsburgh, PA, USA
3 Department of Cell Biology & Physiology, University of Pittsburgh, Pittsburgh, PA, USA
* To whom correspondence should be addressed. E-mail: gla6{at}pitt.edu.
The effect of hydrostatic pressure on ion transport in bladder uroepithelium was investigated. Isolated rabbit uroepithelium was mounted in modified Ussing chambers and mechanically stimulated by applying hydrostatic pressure across the mucosa. Increased hydrostatic pressure led to increased mucosal-to-serosal Na+ absorption across the uroepithelium via the amiloride-sensitive epithelial sodium channel. In addition to this previously characterized pathway for Na+ absorption, hydrostatic pressure also induced Cl- and K+ secretion into the mucosal bathing solution under short-circuit conditions, which was confirmed by a net serosal-to-mucosal flux of 36Cl- and 86Rb+. K+ secretion was via a stretch-activated non-selective cation channel sensitive to 100 µM amiloride, 10 mM tetraethylammonium, 3 mM Ba2+, and 1 mM Gd3+. Hydrostatic pressure-induced ion transport in the uroepithelium may play important roles in electrolyte homeostasis, volume regulation, and mechanosensory transduction.
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