Submitted on October 10, 2007
Accepted on January 16, 2008
Acid, Basic, and Neutral peptidases present different profiles in Chromophobe Renal Cell Carcinoma and in Oncocytoma
Lorena Blanco1, Gorka Larrinaga2, Itxaro Perez1, Jose I Lopez3, Javier Gil4, Ekaitz Agirregoitia5, and Adolfo Varona5*
1 Physiology, University of the Basque Country, Leioa, Vizcaya, Spain; Physiology, University of the Basque Country, Spain
2 Nursing I, University of the Basque Country, Lejona, Vizcaya, Spain
3 Anatomical Pathology, Hospital de Cruces, Baracaldo, Vizcaya, Spain
4 Physiology, University of the Basque Country, Leioa, Vizcaya, Spain
5 Physiology, University of the Basque Country, Lejona, Vizcaya, Spain
* To whom correspondence should be addressed. E-mail: adolfo.varona{at}ehu.es.
Renal Cell Carcinomas (RCCs) are neoplasias with high prevalence and mortality. We previously reported that several peptidases may be involved in the pathophysiology of clear cell renal cell carcinoma (CCRCC). Now, to gain insight into the reasons which lead the various RCC types to behave very differently with regard to aggressiveness and response to anticancer treatments, we analized subsets of chromophobe renal cell carcinoma (ChRCC), and renal oncocytoma (RO), a benign tumor; as well as different grades and stages of CCRCCs.
Particulate APN, APB and APA activities were decreased in both ChRCC and RO (tumor vs non-tumor tissues). Interestingly, activities were down-regulated in a tumor-type specific way and the intensities of the decreases were stronger in the benign tumor than in the malignant type. Moreover, when two key histopathological parameters for tumor prognosis (high vs low stage and grade) were analyzed, also increases of activity were observed in several of these cell-surface peptidases (APN, APB). Some soluble activities (APB, Asp-AP) were also down-regulated in the RCCs. With respect to genetic expression, PSA and APN were in a positive correlation related to their activities in both ChRCC and RO; but not APB, Asp-AP, APA and PGI.
These results may suggest an involvement of several peptidases in the pathophysiology of renal cancer, since they presented different patterns of activity and expression in tumors with different behaviours.
