| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print July 12, 2001
Am J Physiol Renal Physiol, 10.1152/ajprenal.0048.2001
Submitted on February 16, 2001
Accepted on June 16, 2001
1 Medicine, McGill University, Montreal, Quebec, Canada
* To whom correspondence should be addressed. E-mail: ttomok{at}po-box.mcgill.ca.
In rat membranous nephropathy, complement C5b-9 induces glomerular epithelial cell (GEC) injury and proteinuria, which is partially mediated by eicosanoids. Rat GEC in culture express cyclooxygenase (COX)-1 constitutively, while COX-2 expression is induced by C5b-9. Both isoforms contribute to complement-induced prostaglandin generation. The present study addresses mechanisms of complement-induced COX-2 expression in GEC. Downregulation of protein kinase C (PKC) blunted complement-induced upregulation of COX-2 mRNA. Complement and phorbol myristate acetate (PMA) both stimulated COX-2 promoter activity. C5b-9 activated c-Jun NH2-terminal kinase (JNK), and inhibition of JNK activity by transfection of a kinase-inactive JNK1 partially inhibited complement-induced (but not PMA-induced) COX-2 promoter activation. Conversely, a constitutively-active mitogen-activated protein kinase kinase kinase (MEKK)-1, a kinase upstream of JNK, increased COX-2 promoter activity. MEKK-induced COX-2 promoter activation was not affected by downregulation of PKC, and was augmented by PMA. Thus, in GEC, PKC and JNK pathways contribute independently to complement-induced COX-2 expression. Nuclear factor-
B was also activated by complement in GEC, but did not contribute to complement-induced COX-2 upregulation.
This article has been cited by other articles:
|
|
 |
|
  A. V. Cybulsky, T. Takano, J. Guillemette, J. Papillon, R. A. Volpini, and J. A. Di Battista The Ste20-like kinase SLK promotes p53 transactivation and apoptosis Am J Physiol Renal Physiol, October 1, 2009; 297(4): F971 - F980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hussain, L. Romio, M. Saleem, P. Mathieson, M. Serrano, J. Moscat, M. Diaz-Meco, P. Scambler, and A. Koziell Nephrin Deficiency Activates NF-{kappa}B and Promotes Glomerular Injury J. Am. Soc. Nephrol., August 1, 2009; 20(8): 1733 - 1743. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ke, X. Long, Y. Liu, Y.F. Zhang, J. Li, W. Fang, and Q.G. Meng Role of NF-{kappa}B in TNF-{alpha}-induced COX-2 Expression in Synovial Fibroblasts from Human TMJ Journal of Dental Research, April 1, 2007; 86(4): 363 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Cybulsky Cyclooxygenases, Prostanoids, and Glomerular Injury: Complex Relationships J. Am. Soc. Nephrol., February 1, 2007; 18(2): 367 - 368. [Full Text] [PDF]
|
|
|
|
|
|
A. V. Cybulsky, R. J. Quigg, and D. J. Salant Experimental membranous nephropathy redux Am J Physiol Renal Physiol, October 1, 2005; 289(4): F660 - F671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. T. Lappegard, J. Riesenfeld, O.-L. Brekke, G. Bergseth, J. D. Lambris, and T. E. Mollnes Differential Effect of Heparin Coating and Complement Inhibition on Artificial Surface-Induced Eicosanoid Production Ann. Thorac. Surg., March 1, 2005; 79(3): 917 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Aoudjit, M. Stanciu, H. Li, S. Lemay, and T. Takano p38 Mitogen-activated protein kinase protects glomerular epithelial cells from complement-mediated cell injury Am J Physiol Renal Physiol, October 1, 2003; 285(4): F765 - F774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. I. Lemieux, S. S. Rahal, and C. R. J. Kennedy PGE2 reduces arachidonic acid release in murine podocytes: evidence for an autocrine feedback loop Am J Physiol Cell Physiol, February 1, 2003; 284(2): C302 - C309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Peng, T. Takano, J. Papillon, K. Bijian, A. Khadir, and A. V. Cybulsky Complement Activates the c-Jun N-Terminal Kinase/Stress-Activated Protein Kinase in Glomerular Epithelial Cells J. Immunol., September 1, 2002; 169(5): 2594 - 2601. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
