| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print August 15, 2001
Am J Physiol Renal Physiol, 10.1152/ajprenal.0085.2001
Submitted on March 13, 2001
Accepted on August 10, 2001
1 Institute of Anatomy, University of Zurich, Zurich, Switzerland
2 Department of Cellular and Molecular Physiology, Yale University, New Haven, CT, USA
3 Institute of Pharmacology and Toxicology, University of Lausanne, Lausanne, Switzerland
4 Department of Cell Physiology, University Medical Centre Nijmegen, Nijmegen, Netherlands
* To whom correspondence should be addressed. E-mail: jloffing{at}anatom.unizh.ch.
Genetically modified mice are used as models for studying physiology and pathophysiology of electrolyte metabolism, yet, data on the organization of Na+ and Ca2+ transporting proteins along the mouse distal nephron are incomplete. We describe by immunohistochemistry in mouse kidney cortex the sequential distributions of the thiazide-sensitive Na+-Cl--cotransporter (NCC), the amiloride-sensitive epithelial Na+ channel (ENaC), the epithelial Ca2+ channel 1 (ECaC1), the plasma membrane Ca2+-ATPase (PMCA), the Na+-Ca2+ exchanger (NCX), and the cytoplasmic calcium-binding proteins calbindin D28k (CB) and parvalbumin (PV). NCC characterized the distal convoluted tubule (DCT). In the second half of the DCT, in DCT2, NCC was co-expressed with ENaC. The latter extended all along the connecting tubule (CNT) and the cortical collecting duct (CCD). ECaC1 was detected in DCT2 and CNT. The apical abundance of ECaC1 was high in DCT2, decreased, and changed progressively to cytoplasmic location along the CNT. Basolateral NCX and PMCA were weakly apparent in DCT1, were the most abundant in DCT2, decreased gradually along the CNT, and were undetectable in CCD. Cytoplasmic CB was barely detectable in early DCT, steeply increased to very high levels with the beginning of DCT2, and was found at intermediate levels in CNT and CCD. PV was seen at high levels in the cytoplasm and nuclei of DCT1 cells. The co-occurrence of calcium-transporting ECaC1, NCX and PMCA in the mouse DCT2 and CNT reveals the capacity of these segments for active transcellular calcium transport. Co-localization of ECaC1 with NCC and ENaC in the apical plasma membrane of mouse DCT2 cells, and of ECaC1 and ENaC in CNT cells provides a morphological basis for understanding and studying the complex interplay of Na+ and Ca2+ transport in the distal nephron of this species.
This article has been cited by other articles:
|
|
 |
|
  R. T. Alexander, T. E. Woudenberg-Vrenken, J. Buurman, H. Dijkman, B. C. J. van der Eerden, J. P.T.M. van Leeuwen, R. J. Bindels, and J. G. Hoenderop Klotho Prevents Renal Calcium Loss J. Am. Soc. Nephrol., November 1, 2009; 20(11): 2371 - 2379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gamba The thiazide-sensitive Na+-Cl- cotransporter: molecular biology, functional properties, and regulation by WNKs Am J Physiol Renal Physiol, October 1, 2009; 297(4): F838 - F848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Mohebbi, M. Mihailova, and C. A. Wagner The calcineurin inhibitor FK506 (tacrolimus) is associated with transient metabolic acidosis and altered expression of renal acid-base transport proteins Am J Physiol Renal Physiol, August 1, 2009; 297(2): F499 - F509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-S. Lee, K.-C. Choi, and E.-B. Jeung K+-dependent Na+/Ca2+ exchanger 3 is involved in renal active calcium transport and is differentially expressed in the mouse kidney Am J Physiol Renal Physiol, August 1, 2009; 297(2): F371 - F379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Ellison and J. Loffing Thiazide Effects and Adverse Effects: Insights From Molecular Genetics Hypertension, August 1, 2009; 54(2): 196 - 202. [Full Text] [PDF]
|
|
|
|
|
|
M. V. Hofmeister, R. A. Fenton, and J. Praetorius Fluorescence isolation of mouse late distal convoluted tubules and connecting tubules: effects of vasopressin and vitamin D3 on Ca2+ signaling Am J Physiol Renal Physiol, January 1, 2009; 296(1): F194 - F203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang, T. Na, and J.-B. Peng WNK3 positively regulates epithelial calcium channels TRPV5 and TRPV6 via a kinase-dependent pathway Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1472 - F1484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Riveira-Munoz, O. Devuyst, H. Belge, N. Jeck, L. Strompf, R. Vargas-Poussou, X. Jeunemaitre, A. Blanchard, N. V. Knoers, M. Konrad, et al. Evaluating PVALB as a candidate gene for SLC12A3-negative cases of Gitelman's syndrome Nephrol. Dial. Transplant., October 1, 2008; 23(10): 3120 - 3125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Wagner, D. Loffing-Cueni, Q. Yan, N. Schulz, P. Fakitsas, M. Carrel, T. Wang, F. Verrey, J. P. Geibel, G. Giebisch, et al. Mouse model of type II Bartter's syndrome. II. Altered expression of renal sodium- and water-transporting proteins Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1373 - F1380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zacchia and G. Capasso Parvalbumin: a key protein in early distal tubule NaCl reabsorption Nephrol. Dial. Transplant., April 1, 2008; 23(4): 1109 - 1111. [Full Text] [PDF]
|
|
|
|
|
|
J. A. McCormick, C.-L. Yang, and D. H. Ellison WNK Kinases and Renal Sodium Transport in Health and Disease: An Integrated View Hypertension, March 1, 2008; 51(3): 588 - 596. [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. J. Hoenderop and R. J. M. Bindels Calciotropic and Magnesiotropic TRP Channels Physiology, February 1, 2008; 23(1): 32 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wu, X. Gao, R. C. Brown, S. Heller, and R. G. O'Neil Dual role of the TRPV4 channel as a sensor of flow and osmolality in renal epithelial cells Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1699 - F1713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-B. Peng and D. G. Warnock WNK4-mediated regulation of renal ion transport proteins Am J Physiol Renal Physiol, October 1, 2007; 293(4): F961 - F973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Reggiani, D. Raciti, R. Airik, A. Kispert, and A. W. Brandli The prepattern transcription factor Irx3 directs nephron segment identity Genes & Dev., September 15, 2007; 21(18): 2358 - 2370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Mohebbi, J. Kovacikova, M. Nowik, and C. A. Wagner Thyroid hormone deficiency alters expression of acid-base transporters in rat kidney Am J Physiol Renal Physiol, July 1, 2007; 293(1): F416 - F427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Grimm, R. M. Foutz, R. Brenner, and S. C. Sansom Identification and localization of BK-beta subunits in the distal nephron of the mouse kidney Am J Physiol Renal Physiol, July 1, 2007; 293(1): F350 - F359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jiang, W. B. Ferguson, and J.-B. Peng WNK4 enhances TRPV5-mediated calcium transport: potential role in hypercalciuria of familial hyperkalemic hypertension caused by gene mutation of WNK4 Am J Physiol Renal Physiol, February 1, 2007; 292(2): F545 - F554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. J. van de Graaf, J. G. J. Hoenderop, and R. J. M. Bindels Regulation of TRPV5 and TRPV6 by associated proteins Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1295 - F1302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nissant, M. Paulais, S. Lachheb, S. Lourdel, and J. Teulon Similar chloride channels in the connecting tubule and cortical collecting duct of the mouse kidney Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1421 - F1429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Goel, W. G. Sinkins, C.-D. Zuo, M. Estacion, and W. P. Schilling Identification and localization of TRPC channels in the rat kidney Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1241 - F1252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Y. Zhai, J. S. Thomsen, H. Birn, I. B. Kristoffersen, A. Andreasen, and E. I. Christensen Three-Dimensional Reconstruction of the Mouse Nephron J. Am. Soc. Nephrol., January 1, 2006; 17(1): 77 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F.J. van de Graaf, A. W.C.M. van der Kemp, D. van den Berg, M. van Oorschot, J. G.J. Hoenderop, and R. J.M. Bindels Identification of BSPRY as a Novel Auxiliary Protein Inhibiting TRPV5 Activity J. Am. Soc. Nephrol., January 1, 2006; 17(1): 26 - 30. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Picard, M. Van Abel, C. Campone, M. Seiler, M. Bloch-Faure, J. G.J. Hoenderop, J. Loffing, P. Meneton, R. J.M. Bindels, M. Paillard, et al. Tissue Kallikrein-Deficient Mice Display a Defect in Renal Tubular Calcium Absorption J. Am. Soc. Nephrol., December 1, 2005; 16(12): 3602 - 3610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Renkema, T. Nijenhuis, B. C.J. van der Eerden, A. W.C.M. van der Kemp, H. Weinans, J. P.T.M. van Leeuwen, R. J.M. Bindels, and J. G.J. Hoenderop Hypervitaminosis D Mediates Compensatory Ca2+ Hyperabsorption in TRPV5 Knockout Mice J. Am. Soc. Nephrol., November 1, 2005; 16(11): 3188 - 3195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-T. Chen, J. J. Tu, J. Kao, X. K. Zhou, and M. Mazumdar Messenger RNA Expression Ratios among Four Genes Predict Subtypes of Renal Cell Carcinoma and Distinguish Oncocytoma from Carcinoma Clin. Cancer Res., September 15, 2005; 11(18): 6558 - 6566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bazzini, V. Vezzoli, C. Sironi, S. Dossena, A. Ravasio, S. De Biasi, M. Garavaglia, S. Rodighiero, G. Meyer, U. Fascio, et al. Thiazide-sensitive NaCl-cotransporter in the Intestine: POSSIBLE ROLE OF HYDROCHLOROTHIAZIDE IN THE INTESTINAL Ca2+ UPTAKE J. Biol. Chem., May 20, 2005; 280(20): 19902 - 19910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gamba Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters Physiol Rev, April 1, 2005; 85(2): 423 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Jeck, K. P. Schlingmann, S. C. Reinalter, M. Komhoff, M. Peters, S. Waldegger, and H. W. Seyberth Salt handling in the distal nephron: lessons learned from inherited human disorders Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2005; 288(4): R782 - R795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Pluznick, P. Wei, P. R. Grimm, and S. C. Sansom BK-{beta}1 subunit: immunolocalization in the mammalian connecting tubule and its role in the kaliuretic response to volume expansion Am J Physiol Renal Physiol, April 1, 2005; 288(4): F846 - F854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Vallon, P. Wulff, D. Y. Huang, J. Loffing, H. Volkl, D. Kuhl, and F. Lang Role of Sgk1 in salt and potassium homeostasis Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R4 - R10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. J. Hoenderop, B. Nilius, and R. J. M. Bindels Calcium Absorption Across Epithelia Physiol Rev, January 1, 2005; 85(1): 373 - 422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G.J. Hoenderop and R. J.M. Bindels Epithelial Ca2+ and Mg2+ Channels in Health and Disease J. Am. Soc. Nephrol., January 1, 2005; 16(1): 15 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nissant, S. Lourdel, S. Baillet, M. Paulais, P. Marvao, J. Teulon, and M. Imbert-Teboul Heterogeneous distribution of chloride channels along the distal convoluted tubule probed by single-cell RT-PCR and patch clamp Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1233 - F1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Loffing, V. Vallon, D. Loffing-Cueni, F. Aregger, K. Richter, L. Pietri, M. Bloch-Faure, J. G.J. Hoenderop, G. E. Shull, P. Meneton, et al. Altered Renal Distal Tubule Structure and Renal Na+ and Ca2+ Handling in a Mouse Model for Gitelman's Syndrome J. Am. Soc. Nephrol., September 1, 2004; 15(9): 2276 - 2288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Y. Li, C. L. Huey, and A. S. L. Yu Expression of claudin-7 and -8 along the mouse nephron Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1063 - F1071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Y. Huang, P. Wulff, H. Volkl, J. Loffing, K. Richter, D. Kuhl, F. Lang, and V. Vallon Impaired Regulation of Renal K+ Elimination in the sgk1-Knockout Mouse J. Am. Soc. Nephrol., April 1, 2004; 15(4): 885 - 891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. W. Diepens, E. den Dekker, M. Bens, A. F. Weidema, A. Vandewalle, R. J. M. Bindels, and J. G. J. Hoenderop Characterization of a murine renal distal convoluted tubule cell line for the study of transcellular calcium transport Am J Physiol Renal Physiol, March 1, 2004; 286(3): F483 - F489. [Abstract] [Full Text]
|
|
|
|
|
|
P. A. Stehberger, N. Schulz, K. E. Finberg, F. E. Karet, G. Giebisch, R. P. Lifton, J. P. Geibel, and C. A. Wagner Localization and Regulation of the ATP6V0A4 (a4) Vacuolar H+-ATPase Subunit Defective in an Inherited Form of Distal Renal Tubular Acidosis J. Am. Soc. Nephrol., December 1, 2003; 14(12): 3027 - 3038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nijenhuis, J. G. J. Hoenderop, A. W. C. M. van der Kemp, and R. J. M. Bindels Localization and Regulation of the Epithelial Ca2+ Channel TRPV6 in the Kidney J. Am. Soc. Nephrol., November 1, 2003; 14(11): 2731 - 2740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pribanic, S. M. Gisler, D. Bacic, C. Madjdpour, N. Hernando, V. Sorribas, A. Gantenbein, J. Biber, and H. Murer Interactions of MAP17 with the NaPi-IIa/PDZK1 protein complex in renal proximal tubular cells Am J Physiol Renal Physiol, October 1, 2003; 285(4): F784 - F791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-B. Peng, E. M Brown, and M. A Hediger Epithelial Ca2+ entry channels: transcellular Ca2+ transport and beyond J. Physiol., September 15, 2003; 551(3): 729 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Saito, Y. Guan, Z. Qi, L. S. Davis, M. Komhoff, Y. Sugimoto, S. Narumiya, R. M. Breyer, and M. D. Breyer Expression of the prostaglandin F receptor (FP) gene along the mouse genitourinary tract Am J Physiol Renal Physiol, June 1, 2003; 284(6): F1164 - F1170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Frick and D. A. Bushinsky Molecular Mechanisms of Primary Hypercalciuria J. Am. Soc. Nephrol., April 1, 2003; 14(4): 1082 - 1095. [Full Text] [PDF]
|
|
|
|
|
|
J. Loffing and B. Kaissling Sodium and calcium transport pathways along the mammalian distal nephron: from rabbit to human Am J Physiol Renal Physiol, April 1, 2003; 284(4): F628 - F643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Cao, G. Yang, T. L. Timme, T. Saika, L. D. Truong, T. Satoh, A. Goltsov, S. H. Park, T. Men, N. Kusaka, et al. Disruption of the Caveolin-1 Gene Impairs Renal Calcium Reabsorption and Leads to Hypercalciuria and Urolithiasis Am. J. Pathol., April 1, 2003; 162(4): 1241 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bauch, N. Forster, D. Loffing-Cueni, V. Summa, and F. Verrey Functional Cooperation of Epithelial Heteromeric Amino Acid Transporters Expressed in Madin-Darby Canine Kidney Cells J. Biol. Chem., January 3, 2003; 278(2): 1316 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Ferrari, M. G. Bianchetti, A. Sansonnens, and F. J. Frey Modulation of Renal Calcium Handling by 11{beta}-Hydroxysteroid Dehydrogenase Type 2 J. Am. Soc. Nephrol., October 1, 2002; 13(10): 2540 - 2546. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. J. HOENDEROP, O. DARDENNE, M. VAN ABEL, A. W. C. M. VAN DER KEMP, C. H. VAN OS, R. ST.-ARNAUD, and R. J. M. BINDELS Modulation of renal Ca2+ transport protein genes by dietary Ca2+ and 1,25-dihydroxyvitamin D3 in 25-hydroxyvitamin D3-1{alpha}-hydroxylase knockout mice FASEB J, September 1, 2002; 16(11): 1398 - 1406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Magyar, K. E. White, R. Rojas, G. Apodaca, and P. A. Friedman Plasma membrane Ca2+-ATPase and NCX1 Na+/Ca2+ exchanger expression in distal convoluted tubule cells Am J Physiol Renal Physiol, July 1, 2002; 283(1): F29 - F40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Wade Distribution of transporters along the mouse distal nephron: something old, something borrowed, something new Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1019 - F1020. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
