| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print August 9, 2001
Am J Physiol Renal Physiol, 10.1152/ajprenal.0140.2001
Submitted on May 8, 2001
Accepted on June 27, 2001
1 Medicine, University of Florida, Gainesville, Fl, USA
* To whom correspondence should be addressed. E-mail: agarwal{at}nersp.nerdc.ufl.edu.
Heme oxygenase-1 (HO-1) catalyzes the rate limiting step in heme degradation releasing iron, carbon monoxide and biliverdin. Induction of HO-1 occurs as an adaptive and protective response to several inflammatory stimuli. The transcription factor activator protein-1 (AP-1) has been implicated in activation of the HO-1 gene. To elucidate the molecular mechanism of HO-1 induction we examined the effects of curcumin, an inhibitor of the transcription factor AP-1. Surprisingly, curcumin by itself was a very potent inducer of HO-1. Curcumin has anti-inflammatory, antioxidant and renoprotective effects. To evaluate the mechanism of curcumin-mediated induction of HO-1, confluent human renal proximal tubule cells were exposed to curcumin (1-8 µM). We observed a time and dose dependent induction of HO-1 mRNA which was associated with increased HO-1 protein. Co-incubation of curcumin with actinomycin D completely blocked the upregulation of HO-1 mRNA. Blockade of NF-
B, with an IB
phosphorylation inhibitor, attenuated curcumin-mediated induction of HO-1 mRNA and protein. These data demonstrate that curcumin induces HO-1 mRNA and protein in renal proximal tubule cells. HO-1 induction by curcumin is mediated, at least in part, via transcriptional mechanisms and involves the NF-B pathway.
This article has been cited by other articles:
|
|
 |
|
  S. A. Rushworth and D. J. MacEwan HO-1 underlies resistance of AML cells to TNF-induced apoptosis Blood, April 1, 2008; 111(7): 3793 - 3801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Hsieh, C.-S. Rau, M.-W. Hsieh, Y.-C. Chen, S.-F. Jeng, T.-H. Lu, and S.-S. Chen Simvastatin-Induced Heme Oxygenase-1 Increases Apoptosis of Neuro 2A Cells in Response to Glucose Deprivation Toxicol. Sci., January 1, 2008; 101(1): 112 - 121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pugazhenthi, L. Akhov, G. Selvaraj, M. Wang, and J. Alam Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E645 - E655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Alam and J. L. Cook How Many Transcription Factors Does It Take to Turn On the Heme Oxygenase-1 Gene? Am. J. Respir. Cell Mol. Biol., February 1, 2007; 36(2): 166 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Erdmann, N. Grosser, K. Schipporeit, and H. Schroder The ACE Inhibitory Dipeptide Met-Tyr Diminishes Free Radical Formation in Human Endothelial Cells via Induction of Heme Oxygenase-1 and Ferritin J. Nutr., August 1, 2006; 136(8): 2148 - 2152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Goff and H. J. Klee Plant Volatile Compounds: Sensory Cues for Health and Nutritional Value? Science, February 10, 2006; 311(5762): 815 - 819. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Uc, R. F. Husted, R. L. Giriyappa, B. E. Britigan, and J. B. Stokes Hemin induces active chloride secretion in Caco-2 cells Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G202 - G208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Liby, T. Hock, M. M. Yore, N. Suh, A. E. Place, R. Risingsong, C. R. Williams, D. B. Royce, T. Honda, Y. Honda, et al. The Synthetic Triterpenoids, CDDO and CDDO-Imidazolide, Are Potent Inducers of Heme Oxygenase-1 and Nrf2/ARE Signaling Cancer Res., June 1, 2005; 65(11): 4789 - 4798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wesseling, D. A. Ishola Jr., J. A. Joles, H. A. Bluyssen, H. A. Koomans, and B. Braam Resistance to oxidative stress by chronic infusion of angiotensin II in mouse kidney is not mediated by the AT2 receptor Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1191 - F1200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Foresti, M. Hoque, D. Monti, C. J. Green, and R. Motterlini Differential Activation of Heme Oxygenase-1 by Chalcones and Rosolic Acid in Endothelial Cells J. Pharmacol. Exp. Ther., February 1, 2005; 312(2): 686 - 693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hill-Kapturczak, C. Voakes, J. Garcia, G. Visner, H. S. Nick, and A. Agarwal A cis-Acting Region Regulates Oxidized Lipid-Mediated Induction of the Human Heme Oxygenase-1 Gene in Endothelial Cells Arterioscler. Thromb. Vasc. Biol., August 1, 2003; 23(8): 1416 - 1422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Scapagnini, R. Foresti, V. Calabrese, A. M. G. Stella, C. J. Green, and R. Motterlini Caffeic Acid Phenethyl Ester and Curcumin: A Novel Class of Heme Oxygenase-1 Inducers Mol. Pharmacol., March 1, 2002; 61(3): 554 - 561. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
