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Division of Nephrology, School of Medicine, University of Maryland, Baltimore, Maryland 21201-1595
Using nystatin perforated-patch whole
cell recording, we investigated the role of Cl
conductance in the modulation of outer medullary descending vasa recta (OMDVR) pericyte membrane potential (
m) by ANG II. ANG II
(1011 to 107 M) consistently depolarized
OMDVR and induced m oscillations at lower concentrations. The
Cl channel blockers anthracene-9-decarboxylate (1 mM) and
niflumic acid (10 µM) hyperpolarized resting pericytes and
repolarized ANG II-treated pericytes. In voltage-clamp experiments, ANG
II-treated pericytes exhibited slowly activating currents that were
nearly eliminated by treatment with niflumic acid (10 µM) or removal of extracellular Ca2+. Those currents reversed at 
31 and
10 mV when extracellular Cl concentration was 152 and
34 mM, respectively. In pericytes held at 70 mV, oscillating inward
currents were sometimes observed; the reversal potential also shifted
with extracellular Cl concentration. We conclude that ANG
II activates a Ca2+-dependent Cl conductance
in OMDVR pericytes to induce membrane depolarization and m oscillations.
medulla; kidney; microcirculation; patch clamp; niflumic acid; oscillations
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