AJP - Renal Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 283: F73-F85, 2002. First published January 29, 2002; doi:10.1152/ajprenal.00340.2001
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/1/F73    most recent
00340.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gomes, P.
Right arrow Articles by Soares-da-Silva, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gomes, P.
Right arrow Articles by Soares-da-Silva, P.
Vol. 283, Issue 1, F73-F85, July 2002

Expression and function of sodium transporters in two opossum kidney cell clonal sublines

Pedro Gomes1, Jing Xu2, Paula Serrão1, Sofia Dória3, Pedro A. Jose2, and Patrício Soares-da-Silva1

1 Institute of Pharmacology and Therapeutics and 3 Department of Genetics, Faculty of Medicine, 4200-319 Porto, Portugal; and 2 Department of Pediatrics and Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, District of Columbia 20007

The present study describes characteristic features of two clonal subpopulations of opossum kidney (OK) cells (OKLC and OKHC) that are functionally different but morphologically identical. The most impressive differences between OKHC and OKLC cells are the overexpression of Na+-K+-ATPase and type 3 Na+/H+ exchanger by the former, accompanied by an increased Na+-K+-ATPase activity (57.6 ± 5.6 vs. 30.0 ± 0.1 nmol Pi · mg protein-1 · min-1); the increased ability to translocate Na+ from the apical to the basolateral surface; and the increased Na+-dependent pHi recovery (0.254 ± 0.016 vs. 0.094 ± 0.011 pH units/s). Vmax values (in pH units/s) for Na+-dependent pHi recovery in OKHC cells (0.00521 ± 0.0004) were twice (P < 0.05) those in OKLC (0.00202 ± 0.0001), with similar Km values (in mM) for Na+ (OKLC, 21.0 ± 5.5; OKHC, 14.0 ± 5.6). In addition, we measured the activities of transporters (organic ions, alpha -methyl-D-glucoside, L-type amino acids, and Na+) and enzymes (adenylyl cyclase, aromatic L-amino acid decarboxylase, and catechol-O-methyltransferase). The cells were also characterized morphologically by optical and scanning electron microscopy and karyotyped. It is suggested that OKLC and OKHC cells constitute an interesting cell model for the study of renal epithelial physiology and pathophysiology, namely, hypertension.

sodium-hydrogen exchanger; sodium-potassium adenosine 5'-triphosphatase; hypertension


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
E. Efrati, J. Arsentiev-Rozenfeld, and I. Zelikovic
The human paracellin-1 gene (hPCLN-1): renal epithelial cell-specific expression and regulation
Am J Physiol Renal Physiol, February 1, 2005; 288(2): F272 - F283.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Pedrosa, P. Gomes, U. Hopfer, P. A. Jose, and P. Soares-da-Silva
Gi{alpha}3 protein-coupled dopamine D3 receptor-mediated inhibition of renal NHE3 activity in SHR proximal tubular cells is a PLC-PKC-mediated event
Am J Physiol Renal Physiol, November 1, 2004; 287(5): F1059 - F1066.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. J. Pinho, P. Gomes, M. P. Serrao, M. J. Bonifacio, and P. Soares-da-Silva
Organ-Specific Overexpression of Renal LAT2 and Enhanced Tubular L-DOPA Uptake Precede the Onset of Hypertension
Hypertension, October 1, 2003; 42(4): 613 - 618.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online