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Am J Physiol Renal Physiol 283: F1422-F1429, 2002. First published August 21, 2002; doi:10.1152/ajprenal.00095.2002
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Vol. 283, Issue 6, F1422-F1429, December 2002

Nucleotide depletion increases trafficking of gentamicin to the Golgi complex in LLC-PK1 cells

Ruben M. Sandoval1,2, Robert L. Bacallao1,2, Kenneth W. Dunn1, Jeffrey D. Leiser3, and Bruce A. Molitoris1,2

Departments of 1 Medicine and 3 Pediatrics, Divisions of Nephrology, Indiana University School of Medicine, and 2 Roudebush Veterans Administration Medical Center and Indiana Center for Biological Microscopy, Indianapolis, Indiana 46202

Having shown rapid trafficking of aminoglycosides to the Golgi complex in cell culture, we focused on the injurious interaction that occurs when gentamicin administration is preceded by renal ischemia. Using Texas red-labeled gentamicin as a tracer, we determined that 15 min of cellular nucleotide depletion did not significantly increase subsequent uptake. However, cells previously depleted of nucleotides accumulated significantly more Texas red-labeled gentamicin within a dispersed Golgi complex. Using Ricinus communis and Lens culinaris lectins, which label specific compartments of the Golgi complex (trans-Golgi network/trans and medial/cis compartments, respectively), we determined that the medial/cis compartment dispersed after 15 min of nucleotide depletion but the trans-Golgi network/trans compartment remained unaffected. An increase in the number of cells exhibiting disrupted medial/cis-Golgi morphology after repletion in physiological media containing gentamicin was also seen. In summary, the increase in nephrotoxicity seen when ischemia precedes aminoglycoside uptake may be part of a complex mechanism initially involving increased Golgi accumulation and prolonged Golgi dispersion. The Golgi complex must then endure the effects of gentamicin accumulated in larger quantities in an aberrant physiological state.

ATP depletion; ischemia; nephrotoxicity; acute renal failure; proximal tubule cells; cell toxicity; aminoglycosides


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