AJP - Renal Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 284: F411-F417, 2003. First published October 22, 2002; doi:10.1152/ajprenal.00235.2002
0363-6127/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/2/F411    most recent
00235.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schneider, A.
Right arrow Articles by Breyer, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schneider, A.
Right arrow Articles by Breyer, M. D.
Vol. 284, Issue 2, F411-F417, February 2003

SPECIAL COMMUNICATIONS
Differential, inducible gene targeting in renal epithelia, vascular endothelium, and viscera of Mx1Cre mice

André Schneider1, Yahua Zhang2, Youfei Guan2, Linda S. Davis2, and Matthew D. Breyer2,3

1 Universitätsklinikum Eppendorf, 20246 Hamburg, Germany; 2 Division of Nephrology, Department of Medicine, Veterans Affairs Medical Center, and 3 Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37212-2372

The Cre/loxP transgenic system may be used to achieve temporally and/or spatially regulated gene deletion. The Mx1Cre mouse expresses Cre recombinase under control of the IFN-inducible Mx1 promoter. Mx1Cre mice were crossed with a reporter strain (ROSA26tm1Sor) in which beta -galactosidase activity is expressed only after Cre-mediated recombination to determine the cellular pattern of Cre-mediated genetic recombination in the kidney and other tissues. Widespread recombination was observed in vascular endothelium as well as in the liver and spleen. Recombination was restricted to subsets of stromal cells in uterus, duodenum, colon, aorta, and kidney. In the cortex, chi -galactosidase activity was detected in a subset of tubules and all glomerular cells, including endothelium, mesangium, and podocytes. No chi -galactosidase activity was detected in proximal tubules. Costaining of kidneys with segment-specific markers demonstrated induction of chi -galactosidase activity in collecting duct, with sporadic labeling of the thick ascending limb but no significant labeling of distal convoluted tubules. We conclude that Mx1-driven gene recombination is spatially as well as temporally restricted. The Mx1Cre transgene should prove a useful reagent to achieve temporally regulated recombination in endothelial, glomerular, and distal renal epithelia in mice.

Cre recombinase; liver; spleen; interstitium; collecting duct; glomerulus


This article has been cited by other articles:


Home page
 BloodHome page
S. L. Siggins, N.-Y N. Nguyen, M. P. McCormack, S. Vasudevan, R. Villani, S. M. Jane, B. J. Wainwright, and D. J. Curtis
The Hedgehog receptor Patched1 regulates myeloid and lymphoid progenitors by distinct cell-extrinsic mechanisms
Blood, July 30, 2009; 114(5): 995 - 1004.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. S. Morris and A. I. McClatchey
Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma
PNAS, June 16, 2009; 106(24): 9767 - 9772.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. M. Salmon, N. J. Slater, M. A. Hall, M. P. McCormack, S. L. Nutt, S. M. Jane, and D. J. Curtis
Aberrant mast-cell differentiation in mice lacking the stem-cell leukemia gene
Blood, November 15, 2007; 110(10): 3573 - 3581.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. A. Bleuming, X. C. He, L. L. Kodach, J. C. Hardwick, F. A. Koopman, F. J. ten Kate, S. J.H. van Deventer, D. W. Hommes, M. P. Peppelenbosch, G. J. Offerhaus, et al.
Bone Morphogenetic Protein Signaling Suppresses Tumorigenesis at Gastric Epithelial Transition Zones in Mice
Cancer Res., September 1, 2007; 67(17): 8149 - 8155.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
A. Gawlik and S. E. Quaggin
Deciphering the Renal Code: Advances in Conditional Gene Targeting
Physiology, October 1, 2004; 19(5): 245 - 252.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online