HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/1/F68    most recent 00032.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (21) Citing Articles via Google Scholar Google Scholar Articles by Bergeron, M. J. Articles by Isenring, P. Search for Related Content PubMed PubMed Citation Articles by Bergeron, M. J. Articles by Isenring, P.

Ammonium transport and pH regulation by K⁺-Cl^- cotransporters

¹Nephrology Group, Department of Medicine, Faculty of Medicine, Université Laval, Québec G1R 2J6; and ²Groupe de Recherche en Transport Membranaire, Physics Department, Université de Montréal, Montréal, Québec, Canada H3C 3J7

Submitted 22 January 2003 ; accepted in final form 18 March 2003

The Na⁺-K⁺-Cl^- cotransporters (NKCCs), which belong to the cation-Cl^- cotransporter (CCC) family, are able to translocate across cell membranes. In this study, we have used the oocyte expression system to determine whether the K⁺-Cl^- cotransporters (KCCs) can also transport and whether they play a role in pH regulation. Our results demonstrate that all of the CCCs examined (NKCC1, NKCC2, KCC1, KCC3, and KCC4) can promote translocation, presumably through binding of the ion at the K⁺ site. Moreover, kinetic studies for both NKCCs and KCCs suggest that is an excellent surrogate of Rb⁺ or K⁺ and that transport and cellular acidification resulting from CCC activity are relevant physiologically. In this study, we have also found that CCCs are strongly and differentially affected by changes in intracellular pH (independently of intracellular []). Indeed, NKCC2, KCC1, KCC₂, and KCC3 are inhibited at intracellular pH <7.5, whereas KCC4 is activated. These results indicate that certain CCC isoforms may be specialized to operate in acidic environments. CCC-mediated transport could bear great physiological implication given the ubiquitous distribution of these carriers.

NKCC; KCC; ion affinity; acidification; collecting duct

This article has been cited by other articles:

				Y. Kim and L. O. Trussell Negative Shift in the Glycine Reversal Potential Mediated by a Ca2+- and pH-Dependent Mechanism in Interneurons J. Neurosci., September 16, 2009; 29(37): 11495 - 11510. [Abstract] [Full Text] [PDF]

				C. F. Simard, M. J. Bergeron, R. Frenette-Cotton, G. A. Carpentier, M.-E. Pelchat, L. Caron, and P. Isenring Homooligomeric and Heterooligomeric Associations between K+-Cl- Cotransporter Isoforms and between K+-Cl- and Na+-K+-Cl- Cotransporters J. Biol. Chem., June 22, 2007; 282(25): 18083 - 18093. [Abstract] [Full Text] [PDF]

				B. Yang, D. Zhao, E. Solenov, and A. S. Verkman Evidence from knockout mice against physiologically significant aquaporin 8-facilitated ammonia transport Am J Physiol Cell Physiol, September 1, 2006; 291(3): C417 - C423. [Abstract] [Full Text] [PDF]

				M. E. Handlogten, S.-P. Hong, L. Zhang, A. W. Vander, M. L. Steinbaum, M. Campbell-Thompson, and I. D. Weiner Expression of the ammonia transporter proteins Rh B glycoprotein and Rh C glycoprotein in the intestinal tract Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G1036 - G1047. [Abstract] [Full Text] [PDF]

				G. Gamba Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters Physiol Rev, April 1, 2005; 85(2): 423 - 493. [Abstract] [Full Text] [PDF]

				J. R. Williams and J. A. Payne Cation transport by the neuronal K+-Cl- cotransporter KCC2: thermodynamics and kinetics of alternate transport modes Am J Physiol Cell Physiol, October 1, 2004; 287(4): C919 - C931. [Abstract] [Full Text] [PDF]

				M. E. Handlogten, S.-P. Hong, C. M. Westhoff, and I. D. Weiner Basolateral ammonium transport by the mouse inner medullary collecting duct cell (mIMCD-3) Am J Physiol Renal Physiol, October 1, 2004; 287(4): F628 - F638. [Abstract] [Full Text] [PDF]

				E. Gagnon, M. J. Bergeron, G. M. Brunet, N. D. Daigle, C. F. Simard, and P. Isenring Molecular Mechanisms of Cl- Transport by the Renal Na+-K+-Cl- Cotransporter: IDENTIFICATION OF AN INTRACELLULAR LOCUS THAT MAY FORM PART OF A HIGH AFFINITY Cl--BINDING SITE J. Biol. Chem., February 13, 2004; 279(7): 5648 - 5654. [Abstract] [Full Text] [PDF]

				X. Liu, S. Titz, A. Lewen, and U. Misgeld KCC2 Mediates NH4+ Uptake in Cultured Rat Brain Neurons J Neurophysiol, October 1, 2003; 90(4): 2785 - 2790. [Abstract] [Full Text] [PDF]