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Am J Physiol Renal Physiol 285: F664-F673, 2003. First published May 27, 2003; doi:10.1152/ajprenal.00353.2002
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Early transcriptional effects of aldosterone in a mouse inner medullary collecting duct cell line

Michelle L. Gumz,1 Michael P. Popp,2 Charles S. Wingo,3 and Brian D. Cain1

1Department of Biochemistry and Molecular Biology and 2Interdisciplinary Center for Biotechnology Research, University of Florida, and 3Department of Veteran Affairs Medical Center, Gainesville, Florida 32610-0245

Submitted 2 October 2002 ; accepted in final form 21 May 2003

The mineralocorticoid aldosterone is a major regulator of Na+ and acid-base balance and control of blood pressure. Although the long-term effects of aldosterone have been extensively studied, the early aldosterone-responsive genes remain largely unknown. Using DNA array technology, we have characterized changes in gene expression after 1 h of exposure to aldosterone in a mouse inner medullary collecting duct cell line, mIMCD-3. Results from three independent microarray experiments revealed that the expression of many transcripts was affected by aldosterone treatment. Northern blot analysis confirmed the upregulation of four distinct transcripts identified by the microarray analysis, namely, the serum and glucose-regulated kinase sgk, connective tissue growth factor, period homolog, and preproendothelin. Immunoblot analysis for preproendothelin demonstrated increased protein expression. Following the levels of the four transcripts over time showed that each had a unique pattern of expression, suggesting that the cellular response to aldosterone is complex. The results presented here represent a novel list of early aldosterone-responsive transcripts and provide new avenues for elucidating the mechanism of acute aldosterone action in the kidney.

kidney; sgk; period homolog; connective tissue growth factor; endothelin-1


Address for reprint requests and other correspondence: B. D. Cain, Dept. of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 (E-mail: bcain{at}biochem.med.ufl.edu).




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