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INNOVATIVE METHODOLOGY
1Division of Nephrology, Department of Medicine, 2Department of Pathology, and 3Department of Molecular Physiology and Biophysics, Vanderbilt University, and 4Veterans Administration Medical Center, Nashville, Tennessee, 37212
Submitted 9 September 2003 ; accepted in final form 29 October 2003
Two nonradioactive methods for determining glomerular filtration rate (GFR) in conscious mice using FITC-labeled inulin (FITC-inulin) were evaluated. The first method measured GFR using clearance kinetics of plasma FITC-inulin after a single bolus injection. Based on a two-compartment model, estimated GFR was 236.69 ± 16.55 and 140.20 ± 22.27 µl/min in male and female C57BL/6J mice, respectively. Total or 
nephrectomy reduced inulin clearance to 0 or 32.80 ± 9.32 µl/min, respectively. Conversely, diabetes mellitus induced by streptozotocin was associated with increased GFR. The other approach measured urinary inulin clearance using intraperitoneal microosmotic pumps to deliver FITC-inulin and metabolic cages to collect timed urine samples. This approach yielded similar GFR values of 211.11 ± 26.56 and 157.36 ± 20.02 µl/min in male and female mice, respectively. These studies demonstrate the feasibility of repeated nonisotopic measurement of inulin clearance in conscious mice.
hemodynamics; nephrectomy; diabetes mellitus; salts
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