HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/5/F1076    most recent 00075.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (19) Citing Articles via Google Scholar Google Scholar Articles by Song, J. Articles by Ecelbarger, C. A. Search for Related Content PubMed PubMed Citation Articles by Song, J. Articles by Ecelbarger, C. A.

Increased blood pressure, aldosterone activity, and regional differences in renal ENaC protein during vasopressin escape

Division of Endocrinology and Metabolism, Department of Medicine, Georgetown University, Washington, District of Columbia 20057

Submitted 9 March 2004 ; accepted in final form 23 June 2004

The syndrome of inappropriate antidiuretic hormone (SIADH) is associated with water retention and hyponatremia. The kidney adapts via a transient natriuresis and persistent diuresis, i.e., vasopressin escape. Previously, we showed an increase in the whole kidney abundance of aldosterone-sensitive proteins, the - and (70-kDa-band)-subunits of the epithelial Na⁺ channel (ENaC), and the thiazide-sensitive Na-Cl cotransporter (NCC) in our rat model of SIADH. Here we examine mean arterial pressure via radiotelemetry, aldosterone activity, and cortical vs. medullary ENaC subunit and 11-hydroxysteroid dehydrogenase type 2 (11-HSD-2) protein abundances in escape. Eighteen male Sprague-Dawley rats (300 g) were sham operated (n = 6) or infused with desmopressin (dDAVP; n = 12, a V₂ receptor-selective analog of AVP). After 4 days, one-half of the rats receiving dDAVP were switched to a liquid diet, i.e., water loaded (WL) for 5–7 additional days. The WL rats had a sustained increase in urine volume and blood pressure (122 vs. 104 mmHg, P < 0.03, at 7 days). Urine and plasma aldosterone levels were increased in the WL group to 844 and 1,658% of the dDAVP group, respectively. NCC and - and -ENaC (70-kDa band) were increased significantly in the WL group (relative to dDAVP), only in the cortex. - and -ENaC (85-kDa band) were increased significantly by dDAVP in cortex and medulla relative to control. 11-HSD-2 was increased by dDAVP in the cortex and not significantly affected by water loading. These changes may serve to attenuate Na⁺ losses and ameliorate hyponatremia in vasopressin escape.

hyponatremia; syndrome of inappropriate antidiuretic hormone; pressure-natriuresis; renin; 11-hydroxysteroid dehydrogenase; epithelial sodium channel; mean arterial pressure

This article has been cited by other articles:

				Y. H. Kim, T. D. Pham, W. Zheng, S. Hong, C. Baylis, V. Pech, W. H. Beierwaltes, D. B. Farley, L. E. Braverman, J. W. Verlander, et al. Role of pendrin in iodide balance: going with the flow Am J Physiol Renal Physiol, October 1, 2009; 297(4): F1069 - F1079. [Abstract] [Full Text] [PDF]

				S. Riazi, S. Tiwari, N. Sharma, A. Rash, and C. M. Ecelbarger Abundance of the Na-K-2Cl cotransporter NKCC2 is increased by high-fat feeding in Fischer 344 X Brown Norway (F1) rats Am J Physiol Renal Physiol, April 1, 2009; 296(4): F762 - F770. [Abstract] [Full Text] [PDF]

				V. K. Madala Halagappa, S. Tiwari, S. Riazi, X. Hu, and C. M. Ecelbarger Chronic candesartan alters expression and activity of NKCC2, NCC, and ENaC in the obese Zucker rat Am J Physiol Renal Physiol, May 1, 2008; 294(5): F1222 - F1231. [Abstract] [Full Text] [PDF]

				R. F. Husted, K. A. Volk, R. D. Sigmund, and J. B. Stokes Discordant effects of corticosteroids and expression of subunits on ENaC activity Am J Physiol Renal Physiol, September 1, 2007; 293(3): F813 - F820. [Abstract] [Full Text] [PDF]

				S. Tiwari, L. Nordquist, V. K. M. Halagappa, and C. A. Ecelbarger Trafficking of ENaC subunits in response to acute insulin in mouse kidney Am J Physiol Renal Physiol, July 1, 2007; 293(1): F178 - F185. [Abstract] [Full Text] [PDF]

				S. Riazi, O. Khan, X. Hu, and C. A. Ecelbarger Aldosterone infusion with high-NaCl diet increases blood pressure in obese but not lean Zucker rats Am J Physiol Renal Physiol, September 1, 2006; 291(3): F597 - F605. [Abstract] [Full Text] [PDF]

				S. Tiwari, R. K. Packer, X. Hu, Y. Sugimura, J. G. Verbalis, and C. A. Ecelbarger Increased renal {alpha}-ENaC and NCC abundance and elevated blood pressure are independent of hyperaldosteronism in vasopressin escape Am J Physiol Renal Physiol, July 1, 2006; 291(1): F49 - F57. [Abstract] [Full Text] [PDF]

				J. Song, X. Hu, S. Riazi, S. Tiwari, J. B. Wade, and C. A. Ecelbarger Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1055 - F1064. [Abstract] [Full Text] [PDF]

				E. J. Hoorn, J. D. Hoffert, and M. A. Knepper Combined Proteomics and Pathways Analysis of Collecting Duct Reveals a Protein Regulatory Network Activated in Vasopressin Escape J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2852 - 2863. [Abstract] [Full Text] [PDF]

				O. Khan, S. Riazi, X. Hu, J. Song, J. B. Wade, and C. A. Ecelbarger Regulation of the renal thiazide-sensitive Na-Cl cotransporter, blood pressure, and natriuresis in obese Zucker rats treated with rosiglitazone Am J Physiol Renal Physiol, August 1, 2005; 289(2): F442 - F450. [Abstract] [Full Text] [PDF]