HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 292/2/F513    most recent 00222.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Djamali, A. Articles by Becker, B. N. Search for Related Content PubMed PubMed Citation Articles by Djamali, A. Articles by Becker, B. N.

TRANSLATIONAL PHYSIOLOGY

BOLD-MRI assessment of intrarenal oxygenation and oxidative stress in patients with chronic kidney allograft dysfunction

¹Department of Medicine, Section of Nephrology, and Departments of ²Radiology and ³Surgery, University of Wisconsin-Madison, Madison, Wisconsin

Submitted 16 June 2006 ; accepted in final form 16 October 2006

Blood oxygen level-dependent (BOLD) magnetic resonance imaging (MRI) uses deoxyhemoglobin as an endogenous contrast agent for the noninvasive assessment of tissue oxygen bioavailability. We hypothesized that intrarenal oxygenation was impaired in patients with chronic allograft nephropathy (CAN). Ten kidney-transplant recipients with CAN and nine healthy volunteers underwent BOLD-MRI. Medullary R2* (MR2*) and cortical R2* (CR2*) levels (measures directly proportional to tissue deoxyhemoglobin levels) were determined alongside urine and serum markers of oxidative stress (OS): hydrogen peroxide (H₂O₂), F₂-isoprostanes, total nitric oxide (NO), heat shock protein 27 (HSP27), and total antioxidant property (TAOP). Mean MR2* and CR2* levels were significantly decreased in CAN (increased local oxyhemoglobin concentration) compared with healthy volunteers (20.7 ± 1.6 vs. 23.1 ± 1.8/s, P = 0.03 and 15.9 ± 1.9 vs. 13.6 ± 2.3/s, P = 0.05, respectively). There was a significant increase in serum and urine levels of H₂O₂ and serum HSP27 levels in patients with CAN. Conversely, urine NO levels and TAOP were significantly increased in healthy volunteers. Multiple linear regression analyses showed a significant association between MR2* and CR2* levels and serum/urine biomarkers of OS. BOLD-MRI demonstrated significant changes in medullary and cortical oxygen bioavailability in allografts with CAN. These correlated with serum/urine biomarkers of OS, suggesting an association between intrarenal oxygenation and OS.

blood oxygen level-dependent magnetic resonance imaging; deoxyhemoglobin; transplant

This article has been cited by other articles:

				H. Chandarana and V. S. Lee Renal Functional MRI: Are We Ready for Clinical Application? Am. J. Roentgenol., June 1, 2009; 192(6): 1550 - 1557. [Abstract] [Full Text] [PDF]

				R. G. Evans, B. S. Gardiner, D. W. Smith, and P. M. O'Connor Intrarenal oxygenation: unique challenges and the biophysical basis of homeostasis Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1259 - F1270. [Abstract] [Full Text] [PDF]

				H. C. Thoeny, T. M. Kessler, S. Simon-Zoula, F. De Keyzer, M. Mohaupt, U. E. Studer, and P. Vermathen Renal Oxygenation Changes during Acute Unilateral Ureteral Obstruction: Assessment with Blood Oxygen Level-Dependent MR Imaging--Initial Experience Radiology, June 1, 2008; 247(3): 754 - 761. [Abstract] [Full Text] [PDF]

				A. Djamali Oxidative stress as a common pathway to chronic tubulointerstitial injury in kidney allografts Am J Physiol Renal Physiol, August 1, 2007; 293(2): F445 - F455. [Abstract] [Full Text] [PDF]