HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 293/5/F1571    most recent 00132.2007v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Soodvilai, S. Articles by Yang, T. Search for Related Content PubMed PubMed Citation Articles by Soodvilai, S. Articles by Yang, T.

Hydrogen peroxide stimulates chloride secretion in primary inner medullary collecting duct cells via mPGES-1-derived PGE₂

Department of Internal Medicine, University of Utah, and Veterans Affairs Medical Center, Salt Lake City, Utah

Submitted 20 March 2007 ; accepted in final form 30 July 2007

We investigated the role and mechanism of H₂O₂ in regulation of NaCl transport in primary inner medullary collecting duct (IMCD) cells. IMCD cells were isolated from wild-type mice and grown onto semipermeable membranes, and short-circuit current (I_sc) was determined by Ussing chamber. Exposure of IMCD cells to H₂O₂ at a range of 100–300 µM caused a rapid increase in I_sc in a time- and dose-dependent manner. This increase was almost abolished by the cystic fibrosis transmembrane conductance regulator (CFTR) Cl^– channel inhibitors diphenylamine-2-carboxylic acid (DPC) and CFTR_inhibitor-172. In contrast, the magnitude of stimulation was unaffected by the epithelial Na⁺ channel (ENaC) inhibitor amiloride. The H₂O₂-induced Cl^– secretion was significantly inhibited by indomethacin, as well as by microsomal PGE synthase-1 (mPGES-1) deficiency. Like H₂O₂, PGE₂ treatment induced a twofold increase in I_sc that was reduced by the protein kinase A (PKA) inhibitors H-89 and KT5720. These data suggest that H₂O₂ stimulates CFTR Cl^– channel-mediated Cl^– secretion through cyclooxygenase- and mPGES-1-dependent release of PGE₂ and subsequent activation of PKA.

hydrogen peroxide; microsomal prostaglandin E synthase-1; cystic fibrosis transmembrane conductance regulator

This article has been cited by other articles:

				Z. Jia, H. Wang, and T. Yang Mice lacking mPGES-1 are resistant to lithium-induced polyuria Am J Physiol Renal Physiol, December 1, 2009; 297(6): F1689 - F1696. [Abstract] [Full Text] [PDF]