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Nitro-oleic acid protects the mouse kidney from ischemia and reperfusion injury

¹Department of Internal Medicine, University of Utah and Salt Lake Veterans Affairs Medical Center, Salt Lake City; ²Department of Nephrology, 2nd Affiliated Hospital, Shandong University, Jinan, China; Departments of ³Physiology and ⁴Cellular Biology and Anatomy, Medical College of Georgia, and ⁵Medical Research Service, Veterans Affairs Medical Center, Augusta, Georgia; and ⁶College of Health University of Utah, Salt Lake City, Utah

Submitted 3 April 2008 ; accepted in final form 9 June 2008

Nitroalkene derivatives of linoleic acid (nitrolinoleic acid; LNO₂) and nitro-oleic acid (OA-NO₂) are endogenous lipid products with potent anti-inflammatory properties. The present study was undertaken to evaluate the therapeutic potential of OA-NO₂ in a mouse model of renal ischemia-reperfusion (I/R) injury. B6129SF2/J mice were subjected to bilateral renal ischemia for 30 min, followed by 24 h of reperfusion. Fifty minutes after ischemia, mice received intraperitoneal (ip) injections of OA-NO₂ (500 µg/kg; I/R OA-NO₂), vehicle for OA-NO₂ (i.e., 0.8 ml/kg ethanol; I/R veh), or oleic acid (500 µg/kg; I/R OA) every 6 h during the 24-h recovery period. A sham-operated group was not subjected to ischemia and received 0.8 ml/kg ethanol ip every 6 h during the 24-h recovery period (sham veh). While plasma urea and creatinine were elevated (P < 0.05) in I/R veh vs. sham veh mice, the severity was less (P < 0.05) in I/R OA-NO₂ animals. Indices of histological damage, polymorphonucleocyte infiltration, together with expression of intracellular adhesion molecule-1, interleukin-1β, and tumor necrosis factor-, p47^phox, and gp91^phox were greater in I/R veh vs. sham veh mice, but were attenuated (P < 0.05) in I/R OA-NO₂ animals. Because indices of renal dysfunction were similar between I/R veh and I/R OA mice (P > 0.05), but less (P < 0.05) in I/R OA-NO₂ animals compared with both groups, protection from bilateral renal ischemia is afforded by the nitrated but not free form of oleic acid. Together, delayed administration of nitrated fatty acid OA-NO₂ attenuates renal I/R injury in the mouse likely via inhibition of the inflammatory response.

nitrated fatty acids; acute renal failure; inflammation

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