Nitroalkene derivatives of linoleic acid (nitrolinoleic acid, LNO₂) and oleic acid (OA-NO₂) are endogenous lipid products with potent anti-inflammatory properties. The present study was undertaken to evaluate the therapeutic potential of OA-NO₂ a mouse model of renal ischemia/reperfusion (I/R) injury. B6129SF2/J mice were subjected to bilateral renal ischemia for 30-min, followed by 24 h of reperfusion. 50 min after ischemia, mice received intraperitoneal (IP) injections of OA-NO₂ (500 µg/kg; I/R OA-NO₂), vehicle for OA-NO₂ (i.e., 0.8 ml/kg ethanol; I/R veh), or oleic acid (500 µg/kg; I/R OA) every 6 h during the 24 h recovery period. A sham-operated group was not subjected to ischemia and received 0.8 ml/kg ethanol IP every 6 h during the 24 h recovery period (sham veh). While plasma urea and creatinine were elevated (p<0.05) in I/R veh vs. sham veh mice, the severity was less (p<0.05) in I/R OA-NO₂ animals. Indices of histological damage, polymorphonucleocyte infiltration, together with expression of intracellular adhesion molecule-1, interleukin-1, and tumor necrosis factor- activation, p47^phox and gp91^phox were greater in I/R veh vs. sham veh mice, but were attenuated (p<0.05) in I/R OA-NO₂ animals. Because indices of renal dysfunction were similar between I/R veh and I/R OA mice (p>0.05), but less (p<0.05) in I/R OA-NO₂ animals compared to both groups, protection from bilateral renal ischemia is afforded by the nitrated but not free form of oleic acid. Together, delayed administration of nitrated fatty acid OA-NO₂ attenuates renal I/R injury in the mouse likely via inhibition of the inflammatory response.