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Am J Physiol Renal Physiol (November 26, 2008). doi:10.1152/ajprenal.90527.2008
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Submitted on September 4, 2008
Revised on November 13, 2008
Accepted on November 20, 2008

Dietary K regulates ROMK channels in connecting tubule and cortical collecting duct of rat kidney

Gustavo Frindt, Anish Shah1, Johan M. Edvinsson1, and Lawrence G. Palmer1*

1 Weill Medical College of Cornell University

* To whom correspondence should be addressed. E-mail: lgpalm{at}med.cornell.edu.

The activity of ROMK channels in rat kidney epithelial cells was assessed as tertiapin-Q (TPNQ)-sensitive current under whole-cell clamp conditions. With external [K+] = 5 mM and internal [K+] = 140 mM and the membrane potential clamped to 0 mV, TPNQ blocked outward currents in principal cells of the cortical (CCD) and outer-medullary (OMCD) collecting duct and connecting tubule (CNT). The apparent KI was 5.0 nM, consistent with its interaction with ROMK. The TPNQ-sensitive current reversed at voltages close to the equilibrium potential for K+. The currents were reduced when the pipette solution contained ATP. In the CCD, the average TPNQ-sensitive outward current (ISK) was 476 ± 48 pA/cell in control animals on a 1% KCl diet. ISK increased to 1255 ± 140 pA when animals were maintained on a high-K (10% KCl) diet for 7 days, and decreased to 314 ± 46 pA after 7 days on a low-K (0.1% KCl) diet. In the CNT, ISK was 360 ± 30 pA on control, 1160 ± 110 on high-K, and 166 ± 16 pA on low-K diets. The results indicate that ROMK channels activity is highly regulated by dietary K in both the CCD and the CNT.




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