Article Figures & Data
Figures
- Fig. 1.
Effects of the specific inhibitor of CAMKII (KN62) on the large Ca2+-activated K+ channels (BKCa) feedback response to ANG II. A: single BKCa currents (I) in a cell-attached patch showing the response to addition of 1 μM ANG II to the bathing solution. B: effect of 1 μM ANG II on BKCacurrents during exposure to 10 μM KN62. C: bar graph summary of effects of 10 μM KN62 on the ANG II-evoked peakPo of BKCa channels. *P < 0.05 vs. Control.
- Fig. 2.
Effects of KN62 on the Po of BKCa channels in inside-out patches. The bath solution contained 140 mM KCl and 1 μM CaCl2. A: typical tracing showing BKCa currents at a holding potential of 40 mV before (top) and during (bottom) exposure to 10 μM KN62. Arrows indicate closed state. B: results of 6 individual experiments showing the effects of KN62 on thePo of BKCa channels in inside-out patches studied at holding potentials of either 40 mV (●) or −40 mV (○).
- Fig. 3.
Effect of KN62 on the intracellular Ca2+ concentration ([Ca2+]i) response to ANG II. A: typical response of a Control (untreated) cell, to addition of 1 μM ANG II to the bathing solution. B: [Ca2+]i response to ANG II in the presence of 10 μM KN62. C: bar graph summary of the effects of KN62 on the peak and sustained responses to ANG II. ▵, Change.
- Fig. 4.
Effects of CAMKII on BKCa channels in an inside-out patch. The bathing solution contained 140 mM KCl, 10 mM HEPES, and 1 μM Ca2+. Tracings illustrate channel activity under Control conditions (top), after addition of 10 μM calmodulin and 10 μM ATP to the bathing solution (middle), and on subsequent addition of 100 ng/ml CAMKII to the bath.
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