Insulin receptors in podocytes are essential for normal kidney function. Here, we show that insulin evokes a rapid increase in the surface expression of canonical transient receptor potential-6 channel (TRPC6) channels in cultured podocytes, but caused a decrease in surface expression of TRPC5. These effects are accompanied by a marked increase in outwardly rectifying cationic currents that can be blocked by 10 μM SKF96365 or 100 μM La3+. Application of oleoyl-2-acetyl-sn-glycerol (OAG) also increased SKF96365- and La3+-sensitive cationic currents in podocytes. Importantly, current responses to a combination of OAG and insulin were the same amplitude as those evoked by either agent applied alone. This occlusion effect suggests that OAG and insulin are targeting the same population of channels. In addition, shRNA knockdown of TRPC6 markedly reduced cationic currents stimulated by insulin. The effects of insulin on TRPC6 were mimicked by treating podocytes with H2O2. Insulin treatment rapidly increased the generation of H2O2 in podocytes, and it increased the surface expression of the NADPH oxidase NOX4 in cultured podocytes. Basal and insulin-stimulated surface expression of TRPC6 were reduced by pretreatment with diphenylene iodonium, an inhibitor of NADPH oxidases and other flavin-dependent enzymes, by siRNA knockdown of NOX4, and by manganese (III) tetrakis (4-benzoic acid) porphyrin chloride, a membrane-permeable mimetic of superoxide dismutase and catalase. These observations suggest that insulin increases generation of ROS in part through activation of NADPH oxidases, and that this step contributes to modulation of podocyte TRPC6 channels.
- focal and segmental glomerulosclerosis
trpc6 channels are widely expressed Ca2+-permeable cation channels that are activated in the course of G protein and growth factor signaling cascades (12). In 2005 a pair of landmark studies showed that TRPC6 channels are expressed in podocytes and that gain-of-function mutations in TRPC6 channels lead to progressive forms of focal and segmental glomerulosclerosis (FSGS) (44, 55). Additional TRPC6 mutations associated with proteinuric kidney disease have been identified more recently (18), and it was subsequently shown that wild-type TRPC6 channels are expressed at significantly higher levels in acquired proteinuric diseases, including membranous glomerulonephritis and minimal change disease (38). Moreover, selective overexpression of wild-type or mutant TRPC6 channels in mouse podocytes in vivo leads to proteinuria and glomerulosclerosis that resembles human FSGS (31).
TRPC6 channels typically function as downstream plasma membrane components of phospholipase C signaling cascades that liberate diacylglycerol (DAG) and cause local depletion of plasma membrane phosphatidylinositol 4,5-bisphosphate (12). In cultured podocytes, TRPC6 activation can be evoked by angiotensin II (39, 48, 57) and probably by other signals (40). In podocytes, TRPC6 signaling leads to activation of transcription factors such as NFAT (46, 52), and sustained TRPC6 activation in cultured podocytes can induce apoptosis (57). TRPC6 forms part of a larger signaling complex at the glomerular slit diaphragm (12). For example, TRPC6 interacts with the slit diaphragm protein nephrin and its total expression is increased in nephrin knockout mice (44). TRPC6 also interacts with podocin, which may regulate its gating (21), as well as with large-conductance Ca2+-activated K+ (BKCa) channels (26), which may play a role to maintain a driving force for Ca2+ influx in the course of TRPC6 activation (12, 14).
An important recent study showed that selective deletion of insulin receptors in mouse podocytes in vivo causes albuminuria and glomerulosclerosis (53). These knockout mice appeared normal at 3 wk of age, but proteinuria, glomerulosclerosis, foot process effacement, and thickening of the glomerular basement membrane were present by 8 wk of age, as is seen in several mouse models of diabetic nephropathy (5). Moreover, the podocyte-specific insulin receptor knockout mice were normoglycemic (53), indicating that insulin signaling to podocytes is crucial for the normal function of the entire glomerulus. Relatively little is known about the cellular actions of insulin on podocytes. Recent studies showed that insulin stimulates glucose uptake by increasing the cell surface expression of glucose transporters (9, 10). More recently, we showed that insulin also increases surface expression of BKCa channels in podocytes (29).
In this study, we present evidence that insulin modulates TRPC6 channels in cultured podocytes. We observed that treatment of cultured podocytes with insulin causes an increase in the steady-state surface expression of TRPC6 channels that is apparent within minutes, and which is accompanied by an increase in the mean amplitude of outwardly rectifying cationic currents with pharmacological properties that implicate TRPC6 (14, 23). We also show that the effects of insulin on surface expression of TRPC6 are at least partly dependent on generation reactive oxygen species (ROS) such as H2O2, that are generated by NADPH oxidases that include NOX4. Finally, we show that insulin evokes essentially the opposite effect on TRPC5 channels, as we observed a decrease in the steady-state surface expression of those channels after insulin treatment.
MATERIALS AND METHODS
Cell culture protocols, transfection, and drugs.
Cell culture protocols have been described previously (26–30). Briefly, MPC-5 mouse podocyte cell lines (obtained from Dr. Peter Mundel) were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin-streptomycin, with recombinant mouse γ-interferon, in humidified 5% CO2 incubators and propagated at 33°C. Podocyte differentiation and expression of podocyte markers were induced by removal of γ-interferon and temperature switch to 37°C for 14 days. A subclone of the MPC-5 podocyte cell line stably expressing shRNA against TRPC6 described previously (39) was obtained from Dr. Jochen Reiser. Differentiated podocytes were transferred to serum-free media 24 h before experiments with insulin. Cells were then treated with 100 nM recombinant human insulin for various lengths of time (1 min to 72 h) before analyses. A panel of siRNAs directed against NOX4, as well as nontargeted siRNAs for use in control experiments, was obtained from Santa Cruz Biotechnology and transfected into podocytes using Oligofectamine (Invitrogen) in serum-reduced medium according to the manufacturer's directions and as described previously (26–28). Biochemical analyses were carried out 24–48 h after transfection of NOX4 or control siRNA. Recombinant human insulin, 1-oleoyl-2-acetyl-sn-glycerol (OAG), and diphenylene iodonium (DPI) were from Sigma, SKF96365 was from Calbiochem, and manganese (III) tetrakis (4-benzoic acid) porphyrin chloride (MnTBAP) was from Percipio Biosciences.
Immunoblot analysis and cell surface biotinylation assays.
These were carried out as described in detail previously (26–30). Briefly, for immunoblot analyses, proteins in podocyte lysates were separated by SDS-PAGE on 10% gels and transferred to filters. Blots were blocked, incubated with a primary antibody overnight at 4°C, washed again, and the membrane was incubated with horseradish peroxidase (HRP)-conjugated secondary antibody for 1 h at room temperature. Proteins were visualized using a chemiluminescent substrate. The primary antibodies were rabbit anti-TRPC6 (Alomone), mouse monoclonal anti-TRPC5 clone N67/15 (University of California Davis/NIH NeuroMab Facility), rabbit anti-NOX4, and rabbit anti-β-actin (Sigma). For cell surface biotinylation assays, intact podocytes were treated with a membrane-impermeable biotinylation reagent, sulfo-N-hydroxy-succinimidobiotin (Pierce Biotechnology, Rockford, IL; 1 mg/ml) for 1 h. The reaction was stopped, cells were lysed, and biotinylated proteins from the cell surface were recovered by incubation with immobilized streptavidin-agarose beads. A sample of the initial cell lysate was retained for analysis of total proteins. These samples were quantified by immunoblot analysis followed by densitometric analysis using Image J software. Bar graphs describing these data were constructed from three to five repetitions of each experiment.
Assay of ROS generation.
Generation of H2O2 by podocytes was measured using the OxiSelect fluorometric assay (Cell Biolabs). In this assay, H2O2 in the presence of HRP causes oxidation of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) to resorufin, a fluorescent product with a high extinction coefficient (60). Briefly, ADHP/HRP working solution was added to 50-μl samples of serum-free culture supernatant from control or experimental cells according to the manufacturer's directions. Media were then incubated with rocking for 30 min in the dark, and fluorescence was measured with a microplate reader in standard 96-well fluorescence black microtiter plates using excitation at 530 nm and detection at 590 nm. Background fluorescence, measured from culture media that did not contain ADHP, was subtracted before statistical analysis of data.
Whole cell recordings were made using a modification of methods described previously (26–30). Briefly, the bath solution contained 150 mM NaCl, 5.4 mM CsCl, 0.8 mM MgCl2, 5.4 mM CaCl2, and 10 mM HEPES, pH 7.4. Pipette solutions contained 10 mM NaCl, 125 mM CsCl, 6.2 mM MgCl2, 10 mM HEPES, and 10 mM EGTA, pH 7.2. The recording electrodes had resistances of 3–4 MΩ and it was possible to compensate up to 85% of this without introducing oscillations into the current output of the patch-clamp amplifier. Outwardly rectifying currents were evoked by a ramp voltage commands (−80 to + 80 mV over 2.5 s) from a holding potential of −40 mV. Note that there is no K+ in either the bath or pipette solutions, and the dominant cation in the pipette is Cs+, which is designed to prevent contamination from K+ currents. The concentration of Ca2+ in the bath solution (5.4 mM) in all experiments is threefold higher than the normal physiological concentration, by design. After experimenting with many conditions, we found that this helps maintain the stability of whole cell recordings from podocytes, which are flat and relatively fragile. Note, however, that 5.4 mM external Ca2+ markedly reduces inward currents through TRPC6 channels (14, 23) and increases the outward rectification that is a normal feature of these channels under any conditions. For this reason, whole cell currents were quantified at +80 mV. Voltage ramps were applied before and after bath superfusion of 10 μM SKF96365, which produces a rapid and reversible blockade of cation channels in the TRPC family (23, 37). SKF96365-sensitive currents were then obtained by digital subtraction and currents at +80 mV were quantified for statistical analyses. We used a similar procedure in experiments using 100 μM La3+ as an inhibitor of TRPC6 (48). It was not possible to maintain stable recordings long enough to measure currents in a single cell before and after insulin treatments. Therefore, in these experimental designs, statistical analyses were carried out on currents measured from groups of cells (control or insulin-treated). For consistency, we used the same statistical design for experiments with OAG.
Summaries of quantitative data are presented as means ± SE. Electrophysiological data and results of assays for generation of ROS were analyzed by one-way or two-way ANOVA, as indicated, followed by Tukey's post hoc test when multiple groups were compared. We used Student's unpaired t-test in experiments when only two groups were compared. Throughout, P < 0.05 is regarded as significant. For densitometric analyses, immunochemical experiments were run in triplicate, as per standard practice. Means ± SE are provided to illustrate variability of data.
Insulin modulates podocyte TRPC6 channels.
These experiments were performed on differentiated cells of a conditionally immortalized mouse podocyte cell line (MPC-5 cells). After differentiation for 2 wk at 37°C in our laboratory, these cells express the podocyte markers nephrin, Neph1, and synaptopodin (27, 28, 30) and they show physiological responses to insulin (29). We initially examined the effect of insulin on the steady-state surface expression of TRPC6 by means of cell surface biotinylation assays. Differentiated podocytes were maintained in serum-free media for 24 h before insulin treatment. We observed that 100 nM insulin evoked a robust increase in the steady-state surface expression of TRPC6 (Fig. 1). In every experiment, this effect could be seen after 15 min of insulin treatment, and in several experiments this effect was discernible with as little as 1 min of exposure to insulin. However, based on densitometric quantification of these experiments, the largest effects were seen with 24-h or longer insulin treatments, and these effects were typically more than twofold greater than those evoked by 15-min exposures to insulin.
Previous studies on TRPC6 channels in heterologous expression systems showed that their activation gives rise to outwardly rectifying macroscopic cationic currents that are much larger in the presence of DAG analogs (20) and that are reduced in the presence of millimolar external Ca2+ (14, 23). We observed macroscopic currents with these characteristics by means of conventional whole cell recordings from differentiated podocytes (Fig. 2). In these experiments, K+ ions that would normally be present in physiological solutions were replaced by Cs+ in both the bath and pipette solutions. In addition, external Ca2+ was raised to 5.4 mM to improve the stability of whole cell recordings. Macroscopic currents were evoked by 2.5-s voltage ramps from −80 to +80 mV, and currents at +80 mV were quantified for statistical analysis. We observed that cationic currents evoked by voltage ramps under these conditions had an average reversal potential of −4 mV under the recording conditions used in these experiments (N = 18 cells). These currents were substantially larger in cells exposed for 5 min to 100 μM OAG, a membrane-permeable analog of DAG that can activate TRPC6, TRPC3, and TRPC7 channels (14, 20). Moreover, these cationic currents were markedly reduced after superfusion of 10 μM SKF96365, an inhibitor of nonselective cation channels of the TRPC family (37) (Fig. 2, A and B). They were also blocked by superfusion of 100 μM La3+, which blocks TRPC6 channels (48), but increases activation of TRPC5 channels (22, 48, 56) (Fig. 2, C and D). Using a similar design, we observed that treating podocytes with 100 nM insulin could also evoke a significant increase in cationic currents in podocytes that could be instantly blocked by bath superfusion of either 10 μM SKF96365 (Fig. 3, A and B) or 100 μM La3+ (Fig. 3, C and D).
More than one functional member of the TRPC family is expressed in podocytes (12, 48). Because of the lack of selective inhibitors, there is no single experimental design that would allow for completely unambiguous isolation of whole cell currents–or, for that matter, any functional responses–that are mediated by endogenously expressed TRPC6 channels. However, we performed two other experiments that suggest that most or all of the insulin-stimulated current is due to modulation of TRPC6 channels. In one set of experiments, we examined the effects of OAG in control cells and in podocytes previously treated with insulin for 24 h, and we analyzed the results using a two-way ANOVA (Fig. 4A). Currents were recorded from 10 cells in each group (for a total of 40 cells in this experiment). There was a significant effect of both insulin (F1 = 199.38, P < 0.0001) and OAG (F2 = 26.35, P < 0.0005) in the overall data set. Importantly, there was also a statistically significant interaction between these two treatments (F1,2 = 37.74, P < 0.0002). This last result indicates that the response to OAG depends on whether cells were previously treated with insulin. Post hoc analysis indicated that both OAG and insulin were effective when applied by themselves, but that applying OAG to cells immediately after 24-h exposure to insulin treatment did not produce any additional effect. In other words, insulin appears to completely occlude the response to OAG, an observation that is consistent with the hypothesis that OAG and insulin are modulating the same population of channels in podocytes.
Previous studies showed that the set of channels that can be activated by OAG is comprised of TRPC3, TRPC6, and TRPC7 (20). Therefore, in another set of experiments, we examined the effects of OAG and insulin in wild-type podocytes and in a subclone of the same cell line stably expressing an shRNA targeting TRPC6 channels (39). With immunoblot analysis, we observed a ∼75% reduction in total TRPC6 expression in these cells compared with controls (Fig. 4B). We also observed a nearly complete loss of both OAG-evoked cation currents and insulin-stimulated cation currents in the TRPC6 knockdown cells compared with controls (Fig. 4C), even though the knockdown is less than 100%. Our cell surface biotinylation assays indicate that only a fraction of TRPC6 signal, less than 25%, normally reaches the cell surface, even after insulin treatment, and we suspect that the amount of TRPC6 that remains after siRNA knockdown, and which then moves to the cell surface, is not sufficient to give a signal reliably discernible above the normal variance seen in our electrophysiological experiments. Currents were recorded from 10 cells in each group (for a total of 60 cells in this experiment). Analysis of these data by two-way ANOVA revealed significant effects of the TRPC6 knockdown (F1 = 17.18, P < 0.005), as well as significant effects of insulin or OAG treatment (F2 = 66.21, P < 0.0001). Most importantly, there was a highly statistically significant interaction between these two classes of independent variables (F1,2 = 55.47, P < 0.0001). Post hoc analysis indicated that TRPC6 knockdown abolished the ability of podocytes to increase cationic currents in response to either insulin or OAG. Thus, several lines of biochemical and electrophysiological evidence converge to indicate that insulin causes a rapid and functionally significant modulation of TRPC6 channels that is associated with increases in their steady-state expression on the cell surface. In addition, we also examined whether insulin could affect podocyte TRPC5 channels, since this cation channel is also expressed in podocyte cell lines (48). We observed using cell surface biotinylation assays that in marked contrast to its effects on TRPC6, insulin treatment caused a modest decrease in the steady-state surface expression of endogenous TRPC5 channels in podocyte cell lines (data not shown).
Role of ROS and NADPH oxidases.
ROS are now known to play a role in the regulation of a wide range of reversible regulatory processes and in cell signaling (3), including responses to insulin (32, 35). Recent studies also showed that ROS such as H2O2 can increase the amplitude of currents through TRPC6 channels expressed in heterologous expression systems, at least in part owing to rapid increases in TRPC6 expression on the cell surface (16, 52a). We observed that increasing H2O2 can modulate endogenously expressed TRPC6 channels in podocytes (Fig. 5). Thus, simply applying 250 μM or 1 mM H2O2 to podocytes for 30 min caused a robust increase in the steady-state surface expression of TRPC6 as assessed by cell surface biotinylation assays (Fig. 5, A and B). Moreover, insulin treatment increased production of H2O2 by podocytes, with significant effects seen after 15 min of treatment and larger effects observed at 24 h (Fig. 6A). The insulin-evoked increases in ROS generation were blocked by pretreating podocytes with 100 μM MnTBAP, a membrane-permeable mimetic of superoxide dismutase (2) and catalase (13) (Fig. 6B). Importantly, pretreatment with MnTBAP attenuated the increases in surface expression of TRPC6 evoked by 12 h of treatment with 100 nM insulin (Fig. 6, C and D), although we also observed that MnTBAP by itself evoked some increase in surface TRPC6. These data suggest that generation of ROS is necessary for normal insulin-evoked mobilization of TRPC6 channels.
How does insulin evoke generation of ROS in podocytes? In other cells, insulin has been reported to increase ROS by activation of NAPH oxidases (13). There are seven known members of the NOX family (NOX1–5 and DUOX1–2), which are subjected to distinct regulatory cascades (3). Among these, NOX4 is reported to be most heavily expressed in the kidney (15, 47). We detected two molecular weight variants of NOX4 in podocytes; a 66-kDa protein and a larger 75-kDa protein thought to be a glycosylated form (Fig. 7A) (19, 47). Importantly, we observed that insulin treatment caused a strong increase in the surface expression of the larger molecular weight form of NOX4 (Fig. 7, A and B), which is the only form that we detect on the surface. Using siRNA directed against NOX4, we were able to achieve a substantial knockdown of NOX4 (to ∼25% of control levels) as assessed by immunoblot analysis (Fig. 7, C and D). Insulin-evoked modulation of NADPH oxidase appears to be functionally significant, as we observed that basal and insulin-stimulated TRPC6 mobilization was reduced after NOX4 knockdown (Fig. 8, A and B). Similarly, pretreatment with 10 μM DPI, an inhibitor of ROS-generating flavoenzymes such as NOX4 and NOX2 (47, 54), also reduced insulin-evoked mobilization of TRPC6 (Fig. 8, C and D). Collectively, these data suggest that insulin modulation of NADPH oxidases, including NOX4 and possibly other isoforms, is a component of the pathway underlying insulin regulation of TRPC6.
The most important observation in this study is that insulin evokes a marked and rapid increase in the steady-state surface expression of TRPC6 channels in differentiated cells of a podocyte cell line. This effect is at least partially dependent on increased generation of ROS through insulin mobilization of NADPH oxidases, and it is accompanied by an increase in outwardly rectifying cationic currents that can be activated by OAG and blocked by a pan-TRPC channel inhibitor, by micromolar concentrations of La3+, or by knockdown of TRPC6 channels. Finally, we observed that insulin produced a decrease in the steady-state surface expression of endogenous TRPC5 channels, an observation consistent with previous suggestions of a distinct and partially antagonistic role for TRPC5 and TRPC6 channels in the cellular physiology of podocytes (48).
The effect of insulin on podocyte TRPC6 proteins was observed within minutes after application of insulin to serum-starved cells by cell surface biotinylation assays–in some cases in as little as 1 min. A previous study showed that recombinant TRPC6 channels in heterologous expression systems can be modulated by ROS, especially H2O2, which increases TRPC6 channels on the cell surface (16, 52a) and increases their mean open-time (16). We observed similar effects on endogenous TRPC6 channels after treating podocytes with H2O2. There is growing recognition that ROS such as H2O2 and O2·− can function as physiological intracellular messengers, especially in growth factor signaling cascades. ROS can be generated in part through regulated stimulation of NADPH oxidases (3) and generation of these species is a feature of the responses to insulin in several cell types (13, 32, 35). This also occurs after chronic exposure to high glucose (42). The NADPH oxidases comprise a family of homologous flavin-binding transmembrane proteins (NOX1–5 and DUOX1–2) that catalyze transfer of electrons from NADPH to molecular oxygen. These reactions lead typically to production of O2·− (3), which can then be transformed to other ROS, especially the highly lipid-soluble and more persistent H2O2 (41). NOX4 is unusual among the NOX enzymes in that its primary product appears to be H2O2 rather than O2·− (36). NOX4 is also the most abundantly expressed NADPH oxidase in the kidney (15, 47), and we were able to readily detect NOX4 in podocyte cell lines. In vascular endothelial cells, NOX4 is largely localized in intracellular compartments such as endoplasmic reticulum (19). Therefore, it is interesting that we observed a marked increase in the steady-state expression of NOX4 on the surface of podocytes after insulin treatment. To our knowledge, regulated movement of NOX4 to the surface has not been reported in other systems, although newly synthesized NOX4 can be detected by total internal reflectance fluorescence microscopy at the surface of HEK293 cells following tetracycline-induced induction (58). NOX2 is also expressed in podocytes, but we did not observe a change in its location in response to insulin (data not shown), although it was present on the cell surface and could still contribute to insulin-evoked ROS generation in these cells. In this regard, the effects of insulin on TRPC6 were also reduced by the membrane-permeable ROS scavenger MnTBAP and by NOX4 knockdown. They were also reduced by pretreating cells with DPI, which should inhibit catalytic activity of all known NADPH oxidases (54). In summary, these data strongly suggest that insulin activation leads to generation of ROS close to the cell surface, which in turn contributes to modulation of TRPC6 channels of podocytes.
An important recent study showed that insulin signaling in podocytes is essential for normal kidney function (53). This same group also showed that insulin can stimulate insulin uptake into podocytes owing in part to translocation of glucose transporters to the podocyte cell surface (9, 10). The question arises as to what role insulin modulation of podocyte TRPC6 and glucose uptake may play in the integrative physiology of glomerular filtration? One possibility is that an increase in glomerular filtration rate (GFR) is observed in response to intravenous infusions of glucose (6) or after feeding (8, 21a, 49, 51). These stimuli also trigger insulin secretion. This increase in GFR is due in part to increased glomerular transmural pressure gradients driven by proximal tubule glucose reabsorption by the SLGT2 transporter (25, 51). SGLT2 is a Na+-dependent uptake system, and transport of Na+ and glucose by this system will passively drive H2O uptake by proximal tubules. Moreover, the resulting fall in distal tubule Na+ causes dilation of the afferent arteriole owing to inhibition of tubuloglomerular feedback (25, 41, 50, 51). In an intact nephron, one or both of these processes should increase transmural pressure gradients whether the elevated circulating glucose is transient (as in normal subjects following a glucose load) or sustained (as in the early stages of diabetic nephropathy).
Changes in transmural pressure gradients are thought to require mechanical compensation by glomerular cells to maintain the integrity of the filtration barrier (33, 34). We propose that insulin signaling, by increasing surface expression of TRPC6 channels as reported here, and BKCa channels as we reported previously (29), may prepare podocytes to carry out this compensation by allowing for increased Ca2+ influx. Podocytes contract in response to increased Ca2+ influx (45), and this could contribute necessary stability to the glomerular capillary wall–either by helping to maintain the integrity of the slit diaphragm or by preventing podocytes from detaching from the glomerular basement membrane. One point worth noting is that if this model is correct and mobilization of TRPC6 is normally part of a compensatory response to mechanical or metabolic stress of podocytes, then inhibiting these channels may actually be counterproductive in glomerular diseases.
We should note that a recent study examined regulation of TRPC6 channels by H2O2 in cultured human mesangial cells (17). Interestingly, those workers found that application of H2O2 caused a concentration-dependent decrease in total expression of TRPC6–at least after 2–6 h of continuous exposure. They noted that sustained overexpression of NOX4 also reduced total amounts of TRPC6 that they could detect in mesangial cells, whereas knockdown of NOX4 increased total TRPC6 (17). Assuming that measurements of total channel expression reflect expression at the mesangial cell surface, these changes are in the opposite direction of what we observed here for podocytes and what the same group reported earlier for TRPC6 channels in heterologous expression systems (16). One difference is that the mesangial cells were subjected to substantially longer exposures to H2O2 (17). It is possible that ROS has multiple effects that are a function of the concentration and duration of exposure to these species, such that brief exposure causes initial stimulation of TRPC6 channels, but eventually causes a fall in their total expression. However, it is also quite possible that the effects of ROS on TRPC6 are cell-type specific and that a single diffusible mediator could have opposite effects on TRPC6 channels of mesangial cells and podocytes at all times. For example, it is possible that relaxation of mesangial cells (as might be expected from a ROS-induced fall in TRPC6) could increase compliance and allow for some dilation of the capillary tuft, thereby releasing some pressure, while simultaneous ROS-induced activation of TRPC6 in podocytes, as seen here, and after treatment with puromycin aminonucleoside (52a) could promote stability of the slit diaphragm or adhesion of podocytes to the glomerular basement membrane.
In summary, we observed that insulin evokes an increase in the steady-state surface expression of TRPC6 channels in podocytes that depends on NOX-dependent generation of ROS. This response may be part of a mechanism to maintain the stability of the glomerular filtration in the face of stimuli, such as feeding, that are normally associated with changes in renal hemodynamics.
The authors gratefully acknowledge support from National Institutes of Health Grant RO1 DK-82529 and an M. James Scherbenske Grant from the American Society of Nephrology to S. E. Dryer.
No conflicts of interest, financial or otherwise, are declared by the author(s).
Author contributions: E.Y.K. and M.A. performed experiments; E.Y.K. and M.A. analyzed data; E.Y.K. and S.E.D. prepared figures; S.E.D. conception and design of research; S.E.D. interpreted results of experiments; S.E.D. drafted manuscript; S.E.D. edited and revised manuscript; S.E.D. approved final version of manuscript.
We are grateful to Drs. Peter Mundel and Jochen Reiser for supplying podocyte cell lines and to Dr. Anna Greka for essential advice on TRPC5 assays.
- Copyright © 2012 the American Physiological Society