Recent studies suggest that sex of the animal and T cell impacts Ang II hypertension in Rag-/- mice, with females being protected relative to males. This study tested the hypothesis that Ang II results in greater increases in pro-inflammatory T cells and cytokines in males than in females. 12 week old male and female Sprague Dawley (SD) rats were treated with vehicle or Ang II (200 ng/kg/min) for 2 weeks. Renal CD4+ T cells and Tregs were comparable between vehicle-treated males and females, although males expressed more Th17 and IL-17+ T cells and fewer IL-10+ T cells than females. Ang II resulted in greater increases in CD4+ T cells, Th17 cells and IL-17+ cells in males; Tregs increased only in females. We previously showed that Ang (1-7) antagonizes Ang II-induced increases in BP in females and Ang (1-7) has been suggested to be anti-inflammatory. Renal Ang (1-7) levels were greater in female SD at baseline and following Ang II infusion. Additional rats were treated with Ang II plus the Ang (1-7)-mas receptor antagonist A-779 (48 µg/kg/hr) to test the hypothesis that greater Ang (1-7) in females results in more Tregs relative to males. Inhibition of Ang (1-7) did not alter renal T cells in either sex. In conclusion, Ang II induces a sex-specific effect on the renal T cell profile. Males have greater increases in pro-inflammatory T cells, and females have greater increases in anti-inflammatory Tregs, however, sex differences in the renal T cell profile are not mediated by Ang (1-7).
- adaptive immune system
- Sprague Dawley
- Ang (1-7)
- Copyright © 2014, American Journal of Physiology - Renal Physiology